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Virulence Attenuation of Capsular Polysaccharide-deleted Mutants of Streptococcus iniae in Japanese Flounder Paralichthys olivaceus
http://hdl.handle.net/10069/21948
http://hdl.handle.net/10069/219487e21dbc7-e3ce-4d0a-bddb-9e3a5a3b7102
名前 / ファイル | ライセンス | アクション |
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JSFP42_41.pdf (244.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-05-29 | |||||
タイトル | ||||||
タイトル | Virulence Attenuation of Capsular Polysaccharide-deleted Mutants of Streptococcus iniae in Japanese Flounder Paralichthys olivaceus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Streptococcus iniae | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | capsule | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transposon mutagenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | virulence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Paralichthys olivaceus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tn916 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | macrophage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chemiluminescence | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shutou, Kimihiro
× Shutou, Kimihiro× Kanai, Kinya× Yoshikoshi, Kazuma |
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著者別名 | ||||||
姓名 | 首藤, 公宏 | |||||
著者別名 | ||||||
姓名 | 金井, 欣也 | |||||
著者別名 | ||||||
姓名 | 吉越, 一馬 | |||||
その他のタイトル | ||||||
その他のタイトル | Streptococcus iniae 莢膜欠損変異株におけるビルレンスの低下 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Streptococcus iniae is known as an important bacterial pathogen causing serious damage in fish culture industry worldwide. Although the capsule of this pathogen is suggested to play a role in the disease process, it has not been verified yet. In this study we created isogenic uncapsulated mutants of S. iniae NUF631 by Tn916 transposon mutagenesis and investigated the role of capsule in the virulence and resistance to phagocytic activities of peritoneal macrophages of Japanese flounder Paralichthys olivaceus. Mutants transformed from K+ phenotype to K- phenotype were all uncapsulated. They exhibited neither K+ type-specific precipitation line in an immunodiffusion test nor ladder-like banding pattern characteristic for bacterial acidic polysaccharides in polyacrylamide gel electrophoresis. Their virulence to Japanese flounder was highly attenuated. In contrast to the mutant strains, the parent capsulated strain resisted phagocytic and bactericidal activities of the macrophages and prevented chemiluminescence reaction of the macrophages upon phagocytosis. From these results, it is verified that the S. iniae capsule is an important factor for intramacrophage survival of the pathogen. | |||||
書誌情報 |
魚病研究 en : Fish Pathology 巻 42, 号 1, p. 41-48, 発行日 2007-03-15 |
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出版者 | ||||||
出版者 | 日本魚病学会 | |||||
出版者別言語 | ||||||
The Japanese Society of Fish Pathology | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0388788X | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1881-7335 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00063165 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3147/jsfp.42.41 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2007 The Japanese Society of Fish Pathology | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 魚病研究 = Fish Pathology 42(1) pp.41-48; 2007 |