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High-resolution melting analysis for a reliable and two-step scanning of mutations in the tyrosine kinase domain of the chimerical bcr-abl gene.
http://hdl.handle.net/10069/21985
http://hdl.handle.net/10069/21985d251e35e-1317-40cd-a4ab-f60ebace9247
名前 / ファイル | ライセンス | アクション |
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IJH90_37.pdf (704.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-06-12 | |||||
タイトル | ||||||
タイトル | High-resolution melting analysis for a reliable and two-step scanning of mutations in the tyrosine kinase domain of the chimerical bcr-abl gene. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ph | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bcr-abl | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mutation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Melting analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HRM | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Doi, Yuko
× Doi, Yuko× Sasaki, Daisuke× Terada, Chiharu× Mori, Sayaka× Tsuruda, Kazuto× Matsuo, Emi× Miyazaki, Yasushi× Nagai, Kazuhiro× Hasegawa, Hiroo× Yanagihara, Katsunori× Yamada, Yasuaki× Kamihira, Shimeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For relevant imatinib therapy against Philadelphia (Ph)-positive leukemias, it is essential to monitor mutations in the chimerical bcr-abl tyrosine kinase domain (TKD). However, there is no universally acceptable consensus on how to efficiently identify mutations in the target TKD. Recently, high-resolution melting (HRM) technology was developed, which allows gene scanning using an inexpensive generic heteroduplex-detecting dsDNA-binding dye. This study aimed to validate the introduction of HRM in a practical clinical setting for screening of mutations in sporadic sites of the chimerical bcr-abl TKD. All chimerical and wild-type abl TKD regions selectively amplified were used for HRM assays and direct sequencing. The HRM test had approximately 5-90% detection sensitivity for mutations. In contrast to mixture samples with mutant and wild-type cells, all mutant cell samples had indeterminate melting curves equivalent to those of the wild-type due to formation of only a homodulex. This issue was improved by the addition of exogenous wild-type DNA after PCR. Subsequently, HRM results gave a high accordance rate of 97.8% (44/45 samples) compared to the sequencing data. The discordant results in one appear to be due to unsuccessful amplification. Thus, HRM may be considered to be suitable for reliable scanning of mutations in the chimerical abl TKD in a clinical setting. | |||||
書誌情報 |
International journal of hematology 巻 90, 号 1, p. 37-43, 発行日 2009-07 |
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出版者 | ||||||
出版者 | Japanese Society of Hematology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18653774 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10797094 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19466505 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s12185-009-0337-y | |||||
権利 | ||||||
権利情報 | (c)2008 Japanese Society of Hematology | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Hematology, 90(1), pp.37-43; 2009 |