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HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls.
http://hdl.handle.net/10069/22163
http://hdl.handle.net/10069/22163be9b00f8-f8a3-4e24-850b-cc3d579c3905
名前 / ファイル | ライセンス | アクション |
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BT100_4836.pdf (750.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-09-09 | |||||
タイトル | ||||||
タイトル | HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Beta vulgaris | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Metabolic engineering | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | p-Hydroxycinnamoyl-CoA hydratase/lyase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | p-Hydroxybenzoic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell wall | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Rahman, Laiq ur
× Rahman, Laiq ur× Kouno, Hitomi× Hashiguchi, Yuya× Yamamoto, Hirobumi× Narbad, Arjan× Parr, Adrian× Walton, Nicholas× Ikenaga, Toshihiko× Kitamura, Yoshie |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA) at about 14% on a dry weight basis. This is the best yield achieved in plant systems so far. Determination of cell-wall components liberated by alkaline hydrolysis confirmed that the ratio of pHBA to ferulic acid was considerably higher in the HCHL-expressing clones, whereas only ferulic acid was detected in a non-expressing clone. The change in cell-wall components also resulted in a decrease in tensile strength in the HCHL-expressing clones. | |||||
書誌情報 |
Bioresource technology 巻 100, 号 20, p. 4836-4842, 発行日 2009-10 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09608524 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1873-2976 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11522750 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19457657 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.biortech.2009.04.035 | |||||
権利 | ||||||
権利情報 | Copyright c 2009 Elsevier Ltd All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Bioresource technology, 100(20), pp.4836-4842; 2009 |