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Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells.
http://hdl.handle.net/10069/22231
http://hdl.handle.net/10069/22231767324a9-36a7-495f-abe9-f8def254b51b
名前 / ファイル | ライセンス | アクション |
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MRFMMM_Suzuki.pdf (403.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-10-13 | |||||
タイトル | ||||||
タイトル | Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chromosome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In vitro | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Instability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ionizing radiation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Suzuki, Keiji
× Suzuki, Keiji× Kashino, Genro× Kodama, Seiji× Watanabe, Masami |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Ionizing radiation can induce genomic instability in the progeny of irradiated cells, as was demonstrated in various experimental systems. Most in vitro studies have utilized replicating cells, but it is not clear whether radiation-induced genomic instability persists in quiescent cells. Here we show the induction of X-ray-induced genomic instability in normal human diploid cells irradiated and maintained in a quiescent state for up to 24 months while cells were subcultured approximately once every 2-3 months. Every 12 months, a fraction of the irradiated cell population was stimulated to divide by culturing at a low density, and we found that these cells showed increased frequencies of phosphorylated ATM foci, decreased colony-forming ability, and increased frequency of chromosomal aberrations. No significant increases in ROS levels were detected in long-term cultured cells. These results suggest that there are ROS-independent mechanism(s) induced by radiation, which can generate persistent delayed effects in quiescent cells, and could ultimately contribute to carcinogenesis. | |||||
書誌情報 |
Mutation research - Fundamental and Molecular Mechanisms of Mutagenesis 巻 671, 号 1-2, p. 33-39, 発行日 2009-12-01 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00275107 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10747915 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19712688 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.mrfmmm.2009.08.010 | |||||
権利 | ||||||
権利情報 | Copyright c 2009 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Mutation research - Fundamental and Molecular Mechanisms of Mutagenesis, 671(1-2), pp.33-39; 2009 |