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Long-term exposure of human renal carcinoma cells to PD98059 induces epithelial-mesenchymal transition-like phenotype and enhanced motility.
http://hdl.handle.net/10069/22288
http://hdl.handle.net/10069/2228807310069-dbe6-4cd3-bef2-a21c1f719e29
名前 / ファイル | ライセンス | アクション |
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MCB309_69.pdf (573.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-10-23 | |||||
タイトル | ||||||
タイトル | Long-term exposure of human renal carcinoma cells to PD98059 induces epithelial-mesenchymal transition-like phenotype and enhanced motility. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MEK inhibitor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Motility | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Renal carcinoma cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rho | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Stress fiber formation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kanda, Shigeru
× Kanda, Shigeru× Kanetake, Hiroshi× Miyata, Yasuyoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Extracellular signal-regulated kinases (ERK) have fundamental roles in tumor progression. However, human clinical trials have shown little or no effect of inhibitors of their upstream signaling molecule, mitogen-activated protein kinase/ERK kinase (MEK), in advanced cancers. To determine the molecular mechanism underlying the limited antitumor effect, we cultured two human renal carcinoma cell lines, ACHN cells and VMRC-RCW cells in the presence of a MEK inhibitor PD98059 for more than 4 weeks (PD98059-exposed cells). PD98059-exposed ACHN cells showed elongated cell shape with scattering morphology, increase in vimentin expression, loss of beta-catenin junctional localization, stress fiber formation, and increased motility. In contrast, VMRC-RCW cells showed scattered phenotype without PD98059-treatment, and this treatment failed to increase the expression of vimentin. Rho A activity was increased in PD98059-exposed ACHN cells. In these cells, enhanced stress fiber formation and motility were observed, both of which were inhibited by treatment with small interfering RNA for Rho A or an Rho kinase inhibitor Y27632. Our results suggest that long-term exposure of human renal carcinoma cells to PD98059 increases cell motility by upregulating Rho A-Rho kinase signaling. | |||||
書誌情報 |
Molecular and cellular biochemistry 巻 309, 号 1-2, p. 69-76, 発行日 2008-02 |
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出版者 | ||||||
出版者 | Springer Netherlands | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03008177 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1573-4919 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00745800 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18000741 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s11010-007-9644-x | |||||
権利 | ||||||
権利情報 | c Springer Science+Business Media, LLC. 2007 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Molecular and cellular biochemistry, 309(1-2), pp.69-76; 2008 |