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Tum-1, a tumstatin fragment, gene delivery into hepatocellular carcinoma suppresses tumor growth through inhibiting angiogenesis.
http://hdl.handle.net/10069/22339
http://hdl.handle.net/10069/22339b8916ade-5b03-4c4d-8395-edb445128c86
名前 / ファイル | ライセンス | アクション |
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IJO33_33.pdf (301.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-11-09 | |||||
タイトル | ||||||
タイトル | Tum-1, a tumstatin fragment, gene delivery into hepatocellular carcinoma suppresses tumor growth through inhibiting angiogenesis. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tum-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tumstatin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hepatocellular carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | angiogenesis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Goto, Takashi
× Goto, Takashi× Ishikawa, Hiroki× Matsumoto, Kojiro× Nishimura, Daisuke× Kusaba, Mariko× Taura, Naota× Shibata, Hidetaka× Miyaaki, Hisamitsu× Ichikawa, Tatsuki× Hamasaki, Keisuke× Nakao, Kazuhiko× Maeshima, Yohei× Eguchi, Katsumi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Since hepatocellular carcinoma (HCC) is a hypervascular cancer, anti-angiogenic therapy is a promising approach to treat HCC. In the present study, we investigated the antiangiogenic and antitumor effects of tum-1, a fragment of tumstatin, gene transduction into HCC in vitro and in vivo. Tum-1 gene was cloned into a pSecTag2B mammalian expression vehicle to construct pSecTag2B-tum-1. pSecTag2B-tum-1 or vehicle were transfected into human HCC cells, PLC/PRF/5 cells stably and Huh-7 cells tran-siently. pSecTag2B-tum-1 transfection slightly repressed the proliferation of both PLC/PRF/5 and Huh-7 cells in vitro. Addition of conditioned media (CM) from tum-1 expressing PLC/PRF/5 cells significantly inhibited the spontaneous and vascular endothelial growth factor (VEGF)-induced proliferation and migration of human umbilical vein endothelial cells (HUVEC) in vitro with diminishing the VEGF-induced phosphorylation of both Akt and extracellular signal-regulated kinase (ERK) that are known to mediate VEGF-induced proliferation and migration of endothelial cells. In in vivo experiments, intratumoral injection of pSecTag2B-tum-1 significantly repressed the growth of pre-established Huh-7 tumors in athymic mouse models accompanying the decreased density of CD34 positive vessels in tumors. In conclusion, our results suggest that antiangiogenic gene therapy using tum-1 gene may be an efficient strategy for the treatment of HCC. | |||||
書誌情報 |
International Journal of Oncology 巻 33, 号 1, p. 33-40, 発行日 2008-07 |
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出版者 | ||||||
出版者 | Spandidos Publications | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10196439 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1791-2423 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10992511 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18575748 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://hdl.handle.net/10069/26711 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Oncology, 33(1), pp.33-40; 2008 |