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The amount of mineralization after 10 days of irradiation at 2 W for 40 s was significantly increased in comparison to the other conditions (P \u003c 0.05). The extracellular collagen production was significantly increased by 73% on day 2 and 38% on day 4 after laser irradiation (P \u003c 0.05). Although collagen type I gene expression was not changed by laser irradiation, HSP47 gene and protein expression was induced within 12 and 24 h, respectively. CONCLUSIONS: These results suggested that carbon dioxide laser irradiation stimulated mineralization in dental pulp cells. 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Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells.
http://hdl.handle.net/10069/23136
http://hdl.handle.net/10069/23136728db570-647d-4e1b-8661-3bde9d895874
名前 / ファイル | ライセンス | アクション |
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IEJ42_940.pdf (477.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-05-06 | |||||
タイトル | ||||||
タイトル | Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Carbon dioxide laser | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Collagen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dental pulp cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heat shock protein 47 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mineralization | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yasuda, Y
× Yasuda, Y× Ohtomo, E× Tsukuba, Takayuki× Okamoto, K× Saito, T |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | AIM: To examine the effect of carbon dioxide laser irradiation on mineralization in dental pulp cells. METHODOLOGY: Rat dental pulp cells were irradiated with a carbon dioxide laser at 2 W output power for 20, 40 and 60 s, and were cultured in ascorbic acid and beta-glycerophosphate containing media. Cell viability was examined 24 h after laser irradiation by a modified MTT assay. Alizarin Red S staining was performed 10 days after laser irradiation. The amounts of secreted collagen from the cells after irradiation were quantified following Sirius Red staining. The expression levels of collagen type I and HSP47, collagen-binding stress protein, were analysed by real-time PCR. HSP47 protein expression was examined by Western blotting. Statistical analysis was performed using one-way analysis of variance (anova) followed by the Tukey's multiple comparison test. RESULTS: The cell viability was not affected by laser irradiation at 2 W for up to 40 s. However, it was significantly decreased by 20% at 60 s (P < 0.05). The amount of mineralization after 10 days of irradiation at 2 W for 40 s was significantly increased in comparison to the other conditions (P < 0.05). The extracellular collagen production was significantly increased by 73% on day 2 and 38% on day 4 after laser irradiation (P < 0.05). Although collagen type I gene expression was not changed by laser irradiation, HSP47 gene and protein expression was induced within 12 and 24 h, respectively. CONCLUSIONS: These results suggested that carbon dioxide laser irradiation stimulated mineralization in dental pulp cells. The laser irradiation also increased HSP47 expression but not collagen gene expression. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The definitive version is available at www.blackwell-synergy.com | |||||
書誌情報 |
International endodontic journal 巻 42, 号 10, p. 940-946, 発行日 2009-10 |
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出版者 | ||||||
出版者 | Blackwell Publishing Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01432885 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13652591 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00233864 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19751293 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/j.1365-2591.2009.01598.x | |||||
権利 | ||||||
権利情報 | c 2009 International Endodontic Journal | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International endodontic journal, 42(10), pp.940-946; 2009 |