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Multiplex real-time polymerase chain reaction for rapid detection of beta-lactamase-negative, ampicillin-resistant Haemophilus influenzae.
http://hdl.handle.net/10069/22762
http://hdl.handle.net/10069/22762a8f8b4df-1035-46b7-bcc1-ebf567128574
名前 / ファイル | ライセンス | アクション |
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DMID64_72.pdf (390.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-01-26 | |||||
タイトル | ||||||
タイトル | Multiplex real-time polymerase chain reaction for rapid detection of beta-lactamase-negative, ampicillin-resistant Haemophilus influenzae. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BLNAR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Multiplex real-time PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rapid detection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakamura, Shigeki
× Nakamura, Shigeki× Yanagihara, Katsunori× Morinaga, Yoshitomo× Izumikawa, Koichi× Seki, Masafumi× Kakeya, Hiroshi× Yamamoto, Yoshihiro× Kamihira, Shimeru× Kohno, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We evaluated a multiplex real-time quantitative polymerase chain reaction (PCR) method for quantification of Haemophilus influenzae and rapid detection of beta-lactam-resistant strains. We designed 5 PCR primer sets to simultaneously detect the beta-lactam-resistant genes and quantify the pathogen. To demonstrate the validity of this assay, we used 191 clinical isolates, including 141 H. influenzae strains, and 100 purulent sputum samples, including 30 samples from which H. influenzae had been isolated. This assay showed 92.9% sensitivity and 91.8% specificity for detecting beta-lactam-resistant genes, relative to the conventional phenotypic method, and this assay correlated well with conventional quantitative culture counts. By using this assay, we could quantify H. influenzae and identify beta-lactam susceptibility in only 3 h and with only one tube. This method will be helpful for the rapid detection of H. influenzae infections and the selection of appropriate antibiotics. | |||||
書誌情報 |
Diagnostic microbiology and infectious disease 巻 64, 号 1, p. 64-69, 発行日 2009-05 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 07328893 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1879-0070 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10623322 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19232868 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.diagmicrobio.2009.01.023 | |||||
権利 | ||||||
権利情報 | Copyright c 2009 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Diagnostic microbiology and infectious disease, 64(1), pp.64-69; 2009 |