WEKO3
アイテム
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Single Nucleotide Polymorphism that Accompanies a Missense Mutation (Gln488His) Impedes the Dimerization of Hsp90.
http://hdl.handle.net/10069/20950
http://hdl.handle.net/10069/20950c4e5e26b-11d9-46f0-8c42-714b1e7db9ea
名前 / ファイル | ライセンス | アクション |
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ProJou28_24.pdf (350.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-03-04 | |||||
タイトル | ||||||
タイトル | Single Nucleotide Polymorphism that Accompanies a Missense Mutation (Gln488His) Impedes the Dimerization of Hsp90. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dimer formation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hsp90 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Missense mutation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SNP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Trap1 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kobayakawa, Takeshi
× Kobayakawa, Takeshi× Yamada, Shin-Ichi× Mizuno, Akio× Ohara-Nemoto, Yuko× Baba, Tomomi T× Nemoto, Takayuki K |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A single nucleotide polymorphism (SNP) that causes a missense mutation of highly conserved Gln488 to His of the alpha isoform of the 90-kDa heat shock protein (Hsp90alpha) molecular chaperone is observed in Caucasians. The mutated Hsp90alpha severely reduced the growth of yeast cells. To investigate this molecular mechanism, we examined the domain-domain interactions of human Hsp90alpha by using bacterial 2-hybrid system. Hsp90alpha was expressed as a full-length form, N-terminal domain (residues 1-400), or middle (residues 401-617) plus C-terminal (residues 618-732) domains (MC domain/amino acids 401-732). The Gln488His substitution in MC domain did not affect the intra-molecular interaction with N-terminal domain, whereas the dimeric interaction-mediated by the inter-molecular interaction between MC domains was decreased to 32%. Gln488Ala caused a similar change, whereas Gln488Thr, which exceptionally occurs in mitochondrial Hsp90 paralog, fully maintained the dimeric interaction. Therefore, the SNP causing Gln488His mutation could abrogate the Hsp90 function due to reduced dimerization. | |||||
書誌情報 |
The protein journal 巻 28, 号 1, p. 24-28, 発行日 2009-01 |
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出版者 | ||||||
出版者 | Springer Netherlands | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15723887 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1573-4943 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1211041X | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19130193 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10930-008-9160-1 | |||||
権利 | ||||||
権利情報 | (c) Springer Science+Business Media, LLC 2008 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The Protein Journal, 28(1), pp.24-28; 2009 |