WEKO3
アイテム
{"_buckets": {"deposit": "9375c5aa-610d-45f2-9596-db132d1ac1b9"}, "_deposit": {"created_by": 2, "id": "17762", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "17762"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00017762", "sets": ["1330"]}, "author_link": ["70192", "70188", "70190", "70189", "70193", "70191", "70185", "70187", "70186", "70194"], "item_3_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "95", "bibliographicPageStart": "89", "bibliographicVolumeNumber": "53", "bibliographic_titles": [{"bibliographic_title": "Acta medica Nagasakiensia"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Extrahepatic bile duct cancer is a high mortal malignancy. Angiopoietin (Ang) and its receptor Tie, which are known to contribute to angiogenesis, have recently been reported to participate in the proliferation and differentiation of malignant tumor cells. The aim of this study is to investigate the expression and the significance of Ang-1, 2 and Tie-2 in extrahepatic bile duct carcinoma cells. We used immunohistochemistry to study 119 cases of surgically resected human extrahepatic bile duct carcinoma, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) to confirm the expression of Ang-1, 2 and Tie-2 mRNA. Among these 119 cases, 52 (43.7%), 50 (42.0%) and 89 (74.8%) cases showed positive staining for Ang-1, 2 and Tie-2, respectively, in bile duct carcinoma cells. In 38 cases of normal mucosa, 6 (15.8%), 10 (26.3%) and 9 (23.7%) cases were positive for Ang-1, 2 and Tie-2, respectively. The positivity for Ang-1 and Tie-2 in normal mucosa was significantly different from all carcinomas (p\u003c0.01 and p\u003c0.001, respectively). We found no significant correlation between Ang-1 and Ang-2 expression and other clinicopathological factors such as histological differentiation, grade of tumor invasion or survival rate after surgery. In contrast, Tie-2 expression correlated significantly with degree of desmoplasia, cancer stage and survival of patients. RT-PCR analyses of five surgically resected tumor samples and three human bile duct cancer cell lines all showed positive expression of Ang-1, 2 and Tie-2 mRNAs. High expressions of Ang-1, 2 and Tie-2 in human extrahepatic bile duct carcinoma cells suggested that Ang-Tie system may be involved in the progression of human bile duct cancer.", "subitem_description_type": "Abstract"}]}, "item_3_description_64": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Acta medica Nagasakiensia, 53(4), pp.89-95 ; 2009", "subitem_description_type": "Other"}]}, "item_3_relation_42": {"attribute_name": "関係URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/53.89"}]}]}, "item_3_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508430", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00016055", "subitem_source_identifier_type": "ISSN"}]}, "item_3_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mihara, Yumi"}], "nameIdentifiers": [{"nameIdentifier": "70185", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakayama, Toshiyuki"}], "nameIdentifiers": [{"nameIdentifier": "70186", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nanashima, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "70187", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroki, Tamotsu"}], "nameIdentifiers": [{"nameIdentifier": "70188", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Onizuka, Shinya"}], "nameIdentifiers": [{"nameIdentifier": "70189", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "70190", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Naruke, Yuki"}], "nameIdentifiers": [{"nameIdentifier": "70191", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashi, Tomayoshi"}], "nameIdentifiers": [{"nameIdentifier": "70192", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sanefuji, Hayato"}], "nameIdentifiers": [{"nameIdentifier": "70193", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekine, Ichiro"}], "nameIdentifiers": [{"nameIdentifier": "70194", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "acta_53_4_89.pdf", "filesize": [{"value": "169.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 169800.0, "url": {"label": "acta_53_4_89.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/17762/files/acta_53_4_89.pdf"}, "version_id": "0aeb44ec-5a10-4e3f-aa83-8269778d33e3"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Angiopoietin-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Angiopoietin-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tie-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Human bile duct cancer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prognosis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors"}]}, "item_type_id": "3", "owner": "2", "path": ["1330"], "permalink_uri": "http://hdl.handle.net/10069/21633", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-04-22"}, "publish_date": "2009-04-22", "publish_status": "0", "recid": "17762", "relation": {}, "relation_version_is_last": true, "title": ["Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors"], "weko_shared_id": -1}
Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors
http://hdl.handle.net/10069/21633
http://hdl.handle.net/10069/21633b2659cea-a7db-4bd0-ad9d-eaa009a345a4
名前 / ファイル | ライセンス | アクション |
---|---|---|
acta_53_4_89.pdf (169.8 kB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2009-04-22 | |||||
タイトル | ||||||
タイトル | Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Angiopoietin-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Angiopoietin-2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tie-2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human bile duct cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Prognosis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Mihara, Yumi
× Mihara, Yumi× Nakayama, Toshiyuki× Nanashima, Atsushi× Kuroki, Tamotsu× Onizuka, Shinya× Ito, Masahiro× Naruke, Yuki× Hayashi, Tomayoshi× Sanefuji, Hayato× Sekine, Ichiro |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Extrahepatic bile duct cancer is a high mortal malignancy. Angiopoietin (Ang) and its receptor Tie, which are known to contribute to angiogenesis, have recently been reported to participate in the proliferation and differentiation of malignant tumor cells. The aim of this study is to investigate the expression and the significance of Ang-1, 2 and Tie-2 in extrahepatic bile duct carcinoma cells. We used immunohistochemistry to study 119 cases of surgically resected human extrahepatic bile duct carcinoma, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) to confirm the expression of Ang-1, 2 and Tie-2 mRNA. Among these 119 cases, 52 (43.7%), 50 (42.0%) and 89 (74.8%) cases showed positive staining for Ang-1, 2 and Tie-2, respectively, in bile duct carcinoma cells. In 38 cases of normal mucosa, 6 (15.8%), 10 (26.3%) and 9 (23.7%) cases were positive for Ang-1, 2 and Tie-2, respectively. The positivity for Ang-1 and Tie-2 in normal mucosa was significantly different from all carcinomas (p<0.01 and p<0.001, respectively). We found no significant correlation between Ang-1 and Ang-2 expression and other clinicopathological factors such as histological differentiation, grade of tumor invasion or survival rate after surgery. In contrast, Tie-2 expression correlated significantly with degree of desmoplasia, cancer stage and survival of patients. RT-PCR analyses of five surgically resected tumor samples and three human bile duct cancer cell lines all showed positive expression of Ang-1, 2 and Tie-2 mRNAs. High expressions of Ang-1, 2 and Tie-2 in human extrahepatic bile duct carcinoma cells suggested that Ang-Tie system may be involved in the progression of human bile duct cancer. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 53, 号 4, p. 89-95, 発行日 2009-03 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/53.89 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia, 53(4), pp.89-95 ; 2009 |