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A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein.
http://hdl.handle.net/10069/21847
http://hdl.handle.net/10069/21847e400efa2-635d-42fa-97bb-f265e58f63e9
名前 / ファイル | ライセンス | アクション |
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JB_139_4_677.pdf (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-05-13 | |||||
タイトル | ||||||
タイトル | A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hsp70 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | disulfide bridge | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dimer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | client-binding | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nemoto, Takayuki K
× Nemoto, Takayuki K× Fukuma, Yutaka× Itoh, Hideaki× Takagi, Takashi× Ono, Toshio |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The 70-kDa heat shock protein (Hsp70) is predominantly present intracellularly as a monomer, but a small population is converted to dimers and oligomers under certain conditions. In the present study, we investigated the dimeric structure of human inducible Hsp70. As reported earlier, the C-terminal client-binding domain (amino acids 382-641) was required for the dimerization. A 40-amino acid deletion in the client-binding domain from either the N-terminus or C-terminus greatly enhanced the dimerization potential of Hsp70. Limited proteolysis indicated that the dimer formed through truncation from the C-terminus had a conformation similar to that of the non-truncated form. Truncation experiments demonstrated that the client-binding sub-domain (amino acids 382-520) with its adjacent region up to amino acid 541 was not sufficient for the dimerization but that the region up to amino acid 561 was sufficient. Interestingly, the dimer formed through truncation from the C-terminus acquired a homomeric disulfide bridge at Cys574. | |||||
書誌情報 |
Journal of biochemistry 巻 139, 号 4, p. 677-687, 発行日 2006-04 |
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出版者 | ||||||
出版者 | Nihon Seikagakkai | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021924X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00694073 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 16672268 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/jb/mvj071 | |||||
権利 | ||||||
権利情報 | (c) 2006 The Japanese Biochemical Society. | |||||
権利 | ||||||
権利情報 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in "Journal of Biochemistry" following peer review. The definitive publisher-authenticated version "Journal of Biochemistry 2006 139(4):677-687" is available online at: http://jb.oxfordjournals.org/cgi/content/abstract/139/4/677 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of biochemistry. 139(4), pp.677-687; 2006 |