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化学発光計測を利用する分析法
http://hdl.handle.net/10069/21883
http://hdl.handle.net/10069/2188374381913-c9ad-42bf-a2cf-700812cee2fb
名前 / ファイル | ライセンス | アクション |
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BunsekiKagaku49_135.pdf (2.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-05-18 | |||||
タイトル | ||||||
タイトル | 化学発光計測を利用する分析法 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chemiluminescent reagents | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mechanism of chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fluorescent labeling reagent | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPLC | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
中島, 憲一郎
× 中島, 憲一郎 |
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著者別名 | ||||||
姓名 | Nakashima, Kenichiro | |||||
その他のタイトル | ||||||
その他のタイトル | Analytical methods utilizing a chemiluminescence measurement. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 化学反応の結果生じる発光を計測して, 反応基質そのものや反応に関与する化合物を分析する化学発光計測法は, 高感度で選択的な方法の一つとして良く知られている. 特に, 化学発光は高速液体クロマトグラフィー(HPLC)やイムノアッセイの検出系に利用され, 超高感度な分析法が開発されている. 本論文では, 化学発光の歴史について簡単に触れた後で, 代表的な発光反応の機構や特性を概説し, 化学発光計測を利用する分析法について HPLCなどのフロー分析法への応用研究を中心に詳述した. 応用研究については, 化学発光試薬を詳述するとともに, 化学発光計測の特長である高感度性を利用する生体成分などの微量物質の分析例を多数紹介した. また, 新規な化学発光計測あるいは発光試薬を紹介し, それらの分析化学的な応用性について概説した. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Chemiluminescence (CL) is the emission of light as the result of electronic excitation of the luminescing species by a chemical reaction of a precursor of that species. Many CL mechanisms have been studied and utilized to analyze either the chemiluminogen or other compounds that participate in the CL reaction. Generally, the CL detection methods are known to be highly sensitive and selective. Thus, various kinds of CL reagents, such as luminols, acridinium esters, aryloxalates, dioxetanes, and Ru(bpy)_3^<2+>, have been developed and their properties as chemiluminogens examined. Enhancement of the CL reactions has also been examined, and several substances such as 4-iodophenol for luminol-peroxidase-hydrogen peroxide CL, pyrimido[5,4-d]pyrimidines for peroxyoxalate CL and quinine for cerium(IV) CL were found to be useful for this aim. During the last couple of decades, many procedures utilizing CL detection have been developed for determining trace amounts of organic and inorganic compounds, including some of which are biologically important. Among the methods, HPLC or immunoassay with CL detection is more popular and applicable in such fields as clinical and environmental chemistry. Various compounds of biological importance have been determined as diagnosis markers. More recently, capillary electrophoresis with CL detection has become a preferred tool for the analysis of biological samples available in small quantities. In this paper, the application and usage of CL detection in a variety of instrumental analyses, with special emphasis on HPLC, is discussed. | |||||
書誌情報 |
分析化学 en : BUNSEKI KAGAKU 巻 49, 号 3, p. 135-159, 発行日 2000-03 |
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出版者 | ||||||
出版者 | 日本分析化学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05251931 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00222633 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/bunsekikagaku.49.135 | |||||
権利 | ||||||
権利情報 | The Japan Society for Analytical Chemistry (c) 2000 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 分析化学, 49(3), pp. 135-159; 2000 |