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Plectasin has antibacterial activity and no affect on cell viability or IL-8 production
http://hdl.handle.net/10069/19201
http://hdl.handle.net/10069/192011fe37fdb-05e5-4bea-8bc4-d8913f183bc8
名前 / ファイル | ライセンス | アクション |
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BBRC374_709.pdf (2.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-08-21 | |||||
タイトル | ||||||
タイトル | Plectasin has antibacterial activity and no affect on cell viability or IL-8 production | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plectasin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human defensins | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Antibacterial activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytokine induction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytotoxicity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Defensin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human neutrophil peptide-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Interleukin-8 induction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | A549 cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Normal human bronchial epithelial cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Normal human lung fibroblast | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hara, Shintaro
× Hara, Shintaro× Mukae, Hiroshi× Sakamoto, Noriho× Ishimoto, Hiroshi× Amenomori, Misato× Fujita, Hanako× Ishimatsu, Yuji× Yanagihara, Katsunori× Kohno, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Animals and plants express endogenous peptide antibiotics called defensins. Defensins show broad-spectrum antimicrobial activity, even against bacteria that have resistance to conventional antibiotics, which has made them viable candidates for new antibiotics. However, human defensins have failed to reach the market because of their cytotoxic effects and non-antimicrobial bioactivities. Plectasin is a defensin that has shown promise but has not had its potentially negative effects clarified. To address this issue, we examined plectasin’s cytotoxicity in human cells using an AlamarBlue reduction assay, its interleukin (IL)-8-inducing capacity using real-time PCR and ELISA, and measured its MIC against bacteria. We confirmed that plectasin has specific antibacterial activity against Streptococcus pneumoniae. Plectasin showed no cytotoxicity to A549 cells, normal human bronchial epithelial cells, or lung fibroblasts, and it did not induce IL-8 transcription or production in A549 cells. Our results suggest that plectasin could be an inoffensive alternative antibiotic for clinical application. | |||||
書誌情報 |
Biochemical and Biophysical Research Communications 発行日 2008-10-03 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0006291X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00564395 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18675251 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bbrc.2008.07.093 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2008 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biochemical and Biophysical Research Communications, 374(4), pp.709-713; 2008 |