WEKO3
アイテム
{"_buckets": {"deposit": "38c661bb-dfc2-465b-9a73-55d3ab9bc9ef"}, "_deposit": {"created_by": 2, "id": "18205", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "18205"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00018205", "sets": ["9"]}, "author_link": ["72557", "72558", "72556", "72560", "72559", "72553", "72554", "72555"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-07-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "7", "bibliographicPageEnd": "973", "bibliographicPageStart": "966", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Clinical Chemistry and Laboratory Medicine"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: Although mutations in the interferon (IFN) sensitivity determining region (ISDR) of hepatitis C virus (HCV) have been reported to be useful as a predictive viral factor for IFN therapy in patients infected with HCV-1b, such laboratory research has not been favorably translated into the clinic. To promote such translation, we attempted the establishment of a rapid and simple polymerase chain reaction (PCR) combined with melting curve analysis (MCA) to screen for mutations in the ISDR and for the monitoring of HCV quasispecies. Methods: A PCR-MCA protocol was established using in-house primers and hybridization probes designed according to the results of direct sequencing of 34 HCV-1b samples. Then, the performance of PCR-MCA was verified by comparing with mutation profiles obtained by direct sequencing and sequencing after cloning. Results: The MCA assay revealed that melting temperature (Tm) was inversely correlated with the number of nucleotide (nt) and amino acid substitutions in the ISDR deduced on the basis of the results of direct sequencing. A boundary Tm of 58.0°C allowed us to discriminate HCV genomes into two groups: one with a Tm \u003e58.0°C had no or a low number of nt substitutions, while the other genomes with a Tm \u003c58.0°C had a high number of nt substitutions, corresponding to wild-type in the former and mutant-type in the latter in respect of a clinical setting for IFN therapy. Moreover, this MCA assay provided precise discrimination of Tm between clones, reflecting the degree of the genetic complexity of HCV genomes. Conclusions: This study indicates that the MCA assay is useful to rapidly and simply screen the mutational status of the ISDR of HCV, as well as in using the ISDR as one of the targets for discriminating the genetic complexity of HCV genomes. The MCA assay could also be applicable as a convenient and useful screen of the genetic heterogeneity of clones relating to HCV quasispecies.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Clinical Chemistry and Laboratory Medicine, 46(7), pp.966-973; 2008", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Walter de Gruyter"}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "18624619", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1515/CCLM.2008.186", "subitem_relation_type_select": "DOI"}}]}, "item_2_relation_42": {"attribute_name": "関係URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "http://www.degruyter.com/journals/cclm"}]}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright c 2008 Walter de Gruyter."}]}, "item_2_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11209770", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14346621", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sasaki, Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "72553", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugahara, Kazuyuki"}], "nameIdentifiers": [{"nameIdentifier": "72554", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inokuchi, Naoko"}], "nameIdentifiers": [{"nameIdentifier": "72555", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanagihara, Katunori"}], "nameIdentifiers": [{"nameIdentifier": "72556", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasegawa, Hiroo"}], "nameIdentifiers": [{"nameIdentifier": "72557", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Sayaka"}], "nameIdentifiers": [{"nameIdentifier": "72558", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Yasuaki"}], "nameIdentifiers": [{"nameIdentifier": "72559", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamihira, Shimeru"}], "nameIdentifiers": [{"nameIdentifier": "72560", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CCLM46_966.pdf", "filesize": [{"value": "428.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 428600.0, "url": {"label": "CCLM46_966.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/18205/files/CCLM46_966.pdf"}, "version_id": "0100dda0-b04e-4f20-bba1-80545dd96192"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Hepatitis C virus (HCV)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interferon (IFN)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interferon sensitivity determining region (ISDR)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Melting curve analysis (MCA)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Quasispecies", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis"}]}, "item_type_id": "2", "owner": "2", "path": ["9"], "permalink_uri": "http://hdl.handle.net/10069/22065", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-08-04"}, "publish_date": "2009-08-04", "publish_status": "0", "recid": "18205", "relation": {}, "relation_version_is_last": true, "title": ["Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis"], "weko_shared_id": -1}
Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis
http://hdl.handle.net/10069/22065
http://hdl.handle.net/10069/22065a63283ad-b2a0-4eb2-b41c-bc5798e22865
名前 / ファイル | ライセンス | アクション |
---|---|---|
CCLM46_966.pdf (428.6 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2009-08-04 | |||||
タイトル | ||||||
タイトル | Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatitis C virus (HCV) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Interferon (IFN) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Interferon sensitivity determining region (ISDR) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Melting curve analysis (MCA) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Quasispecies | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sasaki, Daisuke
× Sasaki, Daisuke× Sugahara, Kazuyuki× Inokuchi, Naoko× Yanagihara, Katunori× Hasegawa, Hiroo× Mori, Sayaka× Yamada, Yasuaki× Kamihira, Shimeru |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Although mutations in the interferon (IFN) sensitivity determining region (ISDR) of hepatitis C virus (HCV) have been reported to be useful as a predictive viral factor for IFN therapy in patients infected with HCV-1b, such laboratory research has not been favorably translated into the clinic. To promote such translation, we attempted the establishment of a rapid and simple polymerase chain reaction (PCR) combined with melting curve analysis (MCA) to screen for mutations in the ISDR and for the monitoring of HCV quasispecies. Methods: A PCR-MCA protocol was established using in-house primers and hybridization probes designed according to the results of direct sequencing of 34 HCV-1b samples. Then, the performance of PCR-MCA was verified by comparing with mutation profiles obtained by direct sequencing and sequencing after cloning. Results: The MCA assay revealed that melting temperature (Tm) was inversely correlated with the number of nucleotide (nt) and amino acid substitutions in the ISDR deduced on the basis of the results of direct sequencing. A boundary Tm of 58.0°C allowed us to discriminate HCV genomes into two groups: one with a Tm >58.0°C had no or a low number of nt substitutions, while the other genomes with a Tm <58.0°C had a high number of nt substitutions, corresponding to wild-type in the former and mutant-type in the latter in respect of a clinical setting for IFN therapy. Moreover, this MCA assay provided precise discrimination of Tm between clones, reflecting the degree of the genetic complexity of HCV genomes. Conclusions: This study indicates that the MCA assay is useful to rapidly and simply screen the mutational status of the ISDR of HCV, as well as in using the ISDR as one of the targets for discriminating the genetic complexity of HCV genomes. The MCA assay could also be applicable as a convenient and useful screen of the genetic heterogeneity of clones relating to HCV quasispecies. | |||||
書誌情報 |
Clinical Chemistry and Laboratory Medicine 巻 46, 号 7, p. 966-973, 発行日 2008-07-01 |
|||||
出版者 | ||||||
出版者 | Walter de Gruyter | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14346621 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11209770 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18624619 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1515/CCLM.2008.186 | |||||
権利 | ||||||
権利情報 | Copyright c 2008 Walter de Gruyter. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関係URI | ||||||
関連名称 | http://www.degruyter.com/journals/cclm | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Clinical Chemistry and Laboratory Medicine, 46(7), pp.966-973; 2008 |