WEKO3
アイテム
{"_buckets": {"deposit": "e7bc491d-c90c-4d38-9a8f-1e16273e40e5"}, "_deposit": {"created_by": 2, "id": "18238", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "18238"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00018238", "sets": ["28"]}, "author_link": ["72757", "72759", "72758", "72760"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "104", "bibliographicPageStart": "97", "bibliographicVolumeNumber": "13", "bibliographic_titles": [{"bibliographic_title": "Cell Stress and Chaperones"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Two isoforms of the 90-kDa heat-shock protein (Hsp90), i.e., Hsp90alpha and Hsp90beta, are expressed in the cytosol of mammalian cells. Although Hsp90 predominantly exists as a dimer, the dimer-forming potential of the beta isoform of human and mouse Hsp90 is less than that of the alpha isoform. The 16 amino acid substitutions located in the 561-685 amino acid region of the C-terminal dimerization domain should be responsible for this impeded dimerization of Hsp90beta (Nemoto T, Ohara-Nemoto Y, Ota M, Takagi T, Yokoyama K. Eur J Biochem 233: 1-8, 1995). The present study was performed to define the amino acid substitutions that cause the impeded dimerization of Hsp90beta. Bacterial two-hybrid analysis revealed that among the 16 amino acids, the conversion from Ala(558) of Hsp90beta to Thr(566) of Hsp90alpha and that from Met(621) of Hsp90beta to Ala(629) of Hsp90alpha most efficiently reversed the dimeric interaction, and that the inverse changes from those of Hsp90alpha to Hsp90beta primarily explained the impeded dimerization of Hsp90beta We conclude that taken together, the conversion of Thr(566) and Ala(629) of Hsp90alpha to Ala(558) and Met(621) is primarily responsible for impeded dimerization of Hsp90beta.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Cell Stress and Chaperones, 13(1), pp.97-104; 2008", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer Netherlands"}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "18347946", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1007/s12192-008-0017-5", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c Cell Stress Society International 2008"}, {"subitem_rights": "The original publication is available at www.springerlink.com."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13558145", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1466-1268", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kobayakawa, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "72757", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Shin-Ichi"}], "nameIdentifiers": [{"nameIdentifier": "72758", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mizuno, Akio"}], "nameIdentifiers": [{"nameIdentifier": "72759", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nemoto, Takayuki K"}], "nameIdentifiers": [{"nameIdentifier": "72760", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CSC13_97.pdf", "filesize": [{"value": "357.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 357500.0, "url": {"label": "CSC13_97.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/18238/files/CSC13_97.pdf"}, "version_id": "06979a8e-a4b6-4fde-8b04-3d3fda1a93c0"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Hsp90", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Dimerization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Amino acid substitution", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Molecular chaperone", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Substitution of only two residues of human Hsp90alpha causes impeded dimerization of Hsp90beta.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Substitution of only two residues of human Hsp90alpha causes impeded dimerization of Hsp90beta."}]}, "item_type_id": "2", "owner": "2", "path": ["28"], "permalink_uri": "http://hdl.handle.net/10069/20059", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-09-19"}, "publish_date": "2008-09-19", "publish_status": "0", "recid": "18238", "relation": {}, "relation_version_is_last": true, "title": ["Substitution of only two residues of human Hsp90alpha causes impeded dimerization of Hsp90beta."], "weko_shared_id": -1}
Substitution of only two residues of human Hsp90alpha causes impeded dimerization of Hsp90beta.
http://hdl.handle.net/10069/20059
http://hdl.handle.net/10069/2005986ddea7c-4a89-4641-9d0a-28df6ff67309
名前 / ファイル | ライセンス | アクション |
---|---|---|
CSC13_97.pdf (357.5 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-09-19 | |||||
タイトル | ||||||
タイトル | Substitution of only two residues of human Hsp90alpha causes impeded dimerization of Hsp90beta. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hsp90 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dimerization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amino acid substitution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Molecular chaperone | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kobayakawa, Takeshi
× Kobayakawa, Takeshi× Yamada, Shin-Ichi× Mizuno, Akio× Nemoto, Takayuki K |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Two isoforms of the 90-kDa heat-shock protein (Hsp90), i.e., Hsp90alpha and Hsp90beta, are expressed in the cytosol of mammalian cells. Although Hsp90 predominantly exists as a dimer, the dimer-forming potential of the beta isoform of human and mouse Hsp90 is less than that of the alpha isoform. The 16 amino acid substitutions located in the 561-685 amino acid region of the C-terminal dimerization domain should be responsible for this impeded dimerization of Hsp90beta (Nemoto T, Ohara-Nemoto Y, Ota M, Takagi T, Yokoyama K. Eur J Biochem 233: 1-8, 1995). The present study was performed to define the amino acid substitutions that cause the impeded dimerization of Hsp90beta. Bacterial two-hybrid analysis revealed that among the 16 amino acids, the conversion from Ala(558) of Hsp90beta to Thr(566) of Hsp90alpha and that from Met(621) of Hsp90beta to Ala(629) of Hsp90alpha most efficiently reversed the dimeric interaction, and that the inverse changes from those of Hsp90alpha to Hsp90beta primarily explained the impeded dimerization of Hsp90beta We conclude that taken together, the conversion of Thr(566) and Ala(629) of Hsp90alpha to Ala(558) and Met(621) is primarily responsible for impeded dimerization of Hsp90beta. | |||||
書誌情報 |
Cell Stress and Chaperones 巻 13, 号 1, p. 97-104, 発行日 2008-03 |
|||||
出版者 | ||||||
出版者 | Springer Netherlands | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13558145 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1466-1268 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18347946 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s12192-008-0017-5 | |||||
権利 | ||||||
権利情報 | c Cell Stress Society International 2008 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cell Stress and Chaperones, 13(1), pp.97-104; 2008 |