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Detection and assessment of androgenic potency of endocrine-disrupting chemicals using three-spined stickleback, Gasterosteus aculeatus.
http://hdl.handle.net/10069/20083
http://hdl.handle.net/10069/200831fcfce4b-f7c7-415b-aa7e-5090e061b2c2
名前 / ファイル | ライセンス | アクション |
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EnvSci14_255.pdf (342.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-10-09 | |||||
タイトル | ||||||
タイトル | Detection and assessment of androgenic potency of endocrine-disrupting chemicals using three-spined stickleback, Gasterosteus aculeatus. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | stickback | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | spiggin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | endocrine-disrupting chemicals | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biomarker | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | androgen | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nagae, Masaki
× Nagae, Masaki× Kawasaki, Fumie× Tanaka, Yuka× Ohkubo, Nobuyuki× Matsubara, Takahiro× Soyano, Kiyoshi× Hara, Akihiko× Arizono, Koji× Scott, Alexander P× Katsiadaki, Ioanna |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The male three-spined stickleback (Gasterosteus aculeatus) produces a glue protein named "spiggin" that is used as a cementing substance for building its nest. The synthesis of spiggin is under the control of androgenic stimulation. Therefore, spiggin is an effective biomarker of any androgenic activity displayed by environmental chemicals, similarly to the use of vitellogenin as an estrogenic biomarker. The aim of this study was to establish a quantification system for spiggin mRNA to develop a highly sensitive system for evaluating environmental androgens. In this process, two different types of cDNA encoding spiggin (SPG-1 and SPG-2) were isolated. They closely resemble each other in primary structure and features. In addition, the transcriptions of both spiggin gene were induced by only androgenic stimulation in a receptor-mediated manner. These findings suggest the multiplicity albeit specificity of spiggin in the stickleback. The quantification system for spiggin mRNA was established using a real-time RT-PCR technique. This system enables accurate quantification within a wide range of spiggin mRNA from 10(1) to 10(6) copies. | |||||
書誌情報 |
Environmental sciences : an international journal of environmental physiology and toxicology 巻 14, 号 5, p. 255-261, 発行日 2007 |
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出版者 | ||||||
出版者 | MYU | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0915955X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10861036 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 17975537 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://www.myu-inc.jp/myukk/ES/ | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Environmental Sciences, 14(5), pp.255-261; 2007 |