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Usefulness of long-distance inverse polymerase chain reaction for molecular detection of 14q32 translocation in a clinical setting.
http://hdl.handle.net/10069/22530
http://hdl.handle.net/10069/2253039c96748-c682-43a1-98d6-8167c0fb9a6e
名前 / ファイル | ライセンス | アクション |
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SJCLI68_519.pdf (332.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-12-03 | |||||
タイトル | ||||||
タイトル | Usefulness of long-distance inverse polymerase chain reaction for molecular detection of 14q32 translocation in a clinical setting. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | B-cell clonality | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IgH gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lymphoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PCR | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ishizaki(Uemura), Akiko
× Ishizaki(Uemura), Akiko× Sugahara, Kazuyuki× Tsuruda, Kazuto× Hasegawa, Hiroo× Yanagihara, Katsunori× Tsukasaki, Kunihiro× Yamada, Yasuaki× Kamihira, Shimeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | All mature B-cell leukaemias and lymphomas have a clonal Ig gene recombination, and half of them have a reciprocal chromosomal translocation involving the 14q32 locus. The 14q32 translocation partners are variable, such as BCL-2, BCL-1 and BCL-6, thus accounting for the difficulty in molecular detection by the current genomic polymerase chain reaction (PCR) method. To identify B-cell clones efficiently with an Ig gene rearrangement and reciprocal inter-chromosomal translocation, we verified the usefulness, in a practical laboratory setting, of our modified long-distance inverse (LDI) PCR method for detecting IgH gene rearrangements involving inter- and intra-chromosomal segments. The total run time of this LDI PCR method was 5.5 h. Using 24 samples of mature B-cell leukaemias and lymphomas, the modified LDI PCR gave clonally rearranged amplicons in 83 % (20/24) of cases. Direct sequencing results of the amplicons revealed inter-chromosomal translocations in 5 cases (25 %) and intra-chromosomal rearrangements in the remaining 15 cases (75 %). The partners of the inter-chromosomal translocation consisted of the 11q13.3 segment containing a partial BCL1 sequence in 3 cases; 18q21.3 segment containing a partial BCL2 sequence in one case; and a segment of 7q11.2 in one case. We present an LDI PCR-based methodology for the efficient identification of 14q32 translocations, with modifications to reduce the total run time to within one day. | |||||
書誌情報 |
Scandinavian Journal of Clinical and Laboratory Investigation 巻 68, 号 7, p. 519-525, 発行日 2008-11 |
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出版者 | ||||||
出版者 | Informa Healthcare | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00365513 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1502-7686 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18609098 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/00365510701858240 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2008 Informa plc | |||||
権利 | ||||||
権利情報 | This is an electronic version of an article published in Scandinavian Journal of Clinical and Laboratory Investigation, 68(7), 519-525: 2008 November. Scandinavian Journal of Clinical and Laboratory Investigation is available online at: http://www.informaworld.com/openurl?genre=article&issn=00365513&volume=68&issue=7&spage=519. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Scandinavian Journal of Clinical and Laboratory Investigation, 68(7), pp.519-525; 2008 |