WEKO3
アイテム
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These proteins and their export mechanisms are candidates for drug \nand vaccine development, and among them is the Plasmodium SURFIN family of proteins. Previously we showed that the N-terminal region along with the sequence surrounding the transmembrane domain of SURFIN 4.1 is essential for its export to Maurer\u0027s clefts in the red blood cell cytoplasm. We proposed that this region is recognized by a machinery responsible for protein translocation across the parasitophorous vacuole membrane surrounding the parasite. \nTo understand the export mechanism further, we utilized a fluorescent protein-tagged mini-SURFIN 4.1 consisting of the minimum essential components for export. Alanine scanning of all charged amino acids \nwithin the N-terminal region revealed that replacement of 3 glutamic acid and 2 lysine residues significantly impairs the export efficiency of this protein across the parasitophorous vacuole membrane. 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Multiple charged amino acids of Plasmodium falciparum SURFIN4.1 N-terminal region are important for efficient export to the red blood cell
http://hdl.handle.net/10069/39203
http://hdl.handle.net/10069/39203e07c9098-4643-444c-9c58-a99b4f8f19b1
名前 / ファイル | ライセンス | アクション |
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ParInt71_186.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-04-24 | |||||
タイトル | ||||||
タイトル | Multiple charged amino acids of Plasmodium falciparum SURFIN4.1 N-terminal region are important for efficient export to the red blood cell | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Erythrocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Malaria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium falciparum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein export | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SURFIN | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Chitama, Ben-Yeddy Abel
× Chitama, Ben-Yeddy Abel× Miyazaki, Shinya× Zhu, Xiaotong× Kagaya, Wataru× Yahata, Kazuhide× Kaneko, Osamu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Plasmodium falciparum, an obligate intracellular protozoan parasite which causes the severe form of human malaria, exports numerous proteins to the infected red blood cell that are important for its survival and of severe pathological effect to its host. These proteins and their export mechanisms are candidates for drug and vaccine development, and among them is the Plasmodium SURFIN family of proteins. Previously we showed that the N-terminal region along with the sequence surrounding the transmembrane domain of SURFIN 4.1 is essential for its export to Maurer's clefts in the red blood cell cytoplasm. We proposed that this region is recognized by a machinery responsible for protein translocation across the parasitophorous vacuole membrane surrounding the parasite. To understand the export mechanism further, we utilized a fluorescent protein-tagged mini-SURFIN 4.1 consisting of the minimum essential components for export. Alanine scanning of all charged amino acids within the N-terminal region revealed that replacement of 3 glutamic acid and 2 lysine residues significantly impairs the export efficiency of this protein across the parasitophorous vacuole membrane. In addition,N-terminally Myc-tagged mini-SURFIN 4.1 and mini-SURFIN 4.2 with similar architectures were detected with anti-Myc antibody at Maurer's clefts, indicating that elements required for export to Maurer's clefts are conserved between SURFIN 4.1 and SURFIN 4.2 , and that N-terminal sequences of these SURFIN members are not cleaved during export. Our results implicate a conserved nature of SURFIN export to the red blood cell, particularly an important role of multiple glutamic acid and lysine residues in the SURFIN N-terminal region. |
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書誌情報 |
Parasitology International 巻 71, p. 186-193, 発行日 2019-04-24 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13835769 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.parint.2019.04.019 | |||||
権利 | ||||||
権利情報 | c 2019 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Parasitology International, 71, pp.186-193; 2019 |