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INTER-LABORATORY VALIDATION STUDY OF THE SKIN^2 DERMAL MODEL ZK1100 AND MTT CYTOTOXICITY ASSAY KITS
http://hdl.handle.net/10069/18737
http://hdl.handle.net/10069/1873744d55113-4d37-46f4-a83e-efe093fc0d5d
名前 / ファイル | ライセンス | アクション |
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ToSc19_1_37_44.pdf (682.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-07-24 | |||||
タイトル | ||||||
タイトル | INTER-LABORATORY VALIDATION STUDY OF THE SKIN^2 DERMAL MODEL ZK1100 AND MTT CYTOTOXICITY ASSAY KITS | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | the Skin^2 Dermal Model ZK1100 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human keratinocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Inter-laboratory varidation study | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytotoxicity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Alternative to eye irritation test | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Taniguchi, Yuzo
× Taniguchi, Yuzo× Suzuki, Keiji× Nakajima, Keiya× Nakajima, Madoka× Miwa, Yoshihisa× Yamada, Yasushi× Satoh, Matoshi× Takeyoshi, Masahiro× Akie, Yasuki× Moriyasu, Matsuko× Yamamoto, Hitoshi× Katagiri, Minoru× Kazama, Akemi× Watanabe, Masami |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | An inter-laboratory validation study was conducted to evaluate the potential of 4 chemicals to cause irritation with utilizing the Skin^2 Dermal Model ZK1100 kit developed by Advanced Tissue Sciences, Inc. (formerly Marrow-Tech, Inc., La Jolla, California, USA). The chemicals tested were sodium dodecyl sulfate (SDS), 1-n-hexadecyl-pyridinium chloride monohydrate (CC), ethanol (EtOH), and dimethyl sulfoxide (DMSO). Eleven Japanese insititutions participated in this validation research to evaluate the usefulness of the Skin^2 Model ZK1100 kit in accordance with an identical protocol. None of the participating laboratories had previously used the Skin^2 Model ZK1100 kit. The MTT-50 value obtained in the individual institutions was 42 to 91 μg/ml for SDS, 2.7 to 8.6 μg/ml for CC, 2.0 to 9.3% for EtOH, and 11.5 to 21.9% for DMSO. Reproducibility was reasonably good as noted when one test chemical was repetitively tested by the same investigator. MTT-50 values obtained with the present method correlated with DS20 values obtained with Draize's method (r=0.9881) in one of the participant institutions. The irritation study using the Skin^2 Model ZK1100 kit was easy to perform and generated quantitative data. When the test was repeated, reproducibility was demonstrated with a variation of less than 2 6. These data suggested that this newly developed in vitro method would be useful in toxicity screening studies in terms of both time and cost, and would serve as a useful alternative to the conventional methods of the eye irritation study. | |||||
書誌情報 |
Journal of toxicological sciences 巻 19, 号 1, p. 37-44, 発行日 1994-02-25 |
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出版者 | ||||||
出版者 | 日本トキシコロジー学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03881350 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00002808 | |||||
権利 | ||||||
権利情報 | 日本トキシコロジー学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of toxicological sciences. 1994, 19(1), p.37-44 |