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Recombination Activating Gene (RAG)-1 and 2 Encoding Proteins Expressed by the Baculovirus System
http://hdl.handle.net/10069/16049
http://hdl.handle.net/10069/1604955bbcd89-73a8-45d0-98ff-4b42ea55eb55
名前 / ファイル | ライセンス | アクション |
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acta41_01_06_t.pdf (771.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-03-13 | |||||
タイトル | ||||||
タイトル | Recombination Activating Gene (RAG)-1 and 2 Encoding Proteins Expressed by the Baculovirus System | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Araki, Ryoko
× Araki, Ryoko× Kaku, Mitsuo× Itoh, Masahiro× Hamatani, Kiyohiro× Usui, Toshiaki× Abe, Masumi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have been attempting to obtain mouse recombination activating gene-1 (RAG-1) and RAG-2 protein for biochemical analyses. First of all, we obtained truncated products of these genes expressed and purified using the E. coli expression system and then established the polyclonal and monoclonal antibodies by means of E. coli expressed peptides as antigens. Subsequently, whole RAG-1 and RAG-2 gene products were expressed the baculovirus expression system. Since it has been difficult to achieve the significant gene expression of full-lenght cDNA, we employed the glutathione S-transferase (GST)-fused gene-expression system which facilitated the massive expression of gene products. This system was also advantageous in that we could detect the expressed protein molecules not only with anti-RAG antibody but also with anti-GST antibody. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 41, 号 1-2, p. 43-47, 発行日 1996-06-20 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00043-P00047 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1996, 41(1-2), p.43-47 |