WEKO3
アイテム
{"_buckets": {"deposit": "10b4aa9c-1e85-4e46-81a0-ce69a367ebba"}, "_deposit": {"created_by": 2, "id": "20106", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "20106"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00020106", "sets": ["1389"]}, "author_link": ["84737", "84736", "84738"], "item_3_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-06-20", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "57", "bibliographicPageStart": "53", "bibliographicVolumeNumber": "41", "bibliographic_titles": [{"bibliographic_title": "Acta medica Nagasakiensia"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The amino acid sequence of a cyanogen bromide-generated peptide corresponding to the region of subfragment-1 of chicken gizzard myosin, predicted from the cDNA sequence to contain a seven-amino acid insertion, was determined directly. Subfragment-1 was prepared from adult chicken gizzard myosin by limited digestion with papain, and a 137-residue peptide was obtained by cleaving subfragment-1 with cyanogen bromide. Amino acid composition analysis and sequence analysis of the fragments generated from this peptide by formic acid or a-chymotrypsin revealed that the peptide corresponded to leucine-165 to methionine-301 of chicken gizzard myosin heavy chain previously deduced from the cDNA sequence by Yanagisawa, M. et al.. The predicted seven-amino acid insertion (Gln-Gly-Pro-Ser-Phe-Ser-Tyr), absent from other smooth muscle myosin,was thus confirmed to be present in chicken gizzard myosin. The amino acid sequence surrounding the insertion site of chicken gizzard myosin shows greater homology to the corresponding sequence of other known smooth muscle and non-muscle myosins than to those of chicken skeletal and cardiac muscle myosins. The insertion sequence may confer gizzard-specific functions to the gizzard myosin heavy chain, whereas the amino acid sequence surrounding the insertion site may be specific to smooth muscle.", "subitem_description_type": "Abstract"}]}, "item_3_description_64": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Acta medica Nagasakiensia. 1996, 41(1-2), p.53-57", "subitem_description_type": "Other"}]}, "item_3_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508430", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00016055", "subitem_source_identifier_type": "ISSN"}]}, "item_3_text_62": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "P00053-P00057"}]}, "item_3_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sakurai, Masaharu"}], "nameIdentifiers": [{"nameIdentifier": "84736", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Bunji"}], "nameIdentifiers": [{"nameIdentifier": "84737", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagata, Shuichi"}], "nameIdentifiers": [{"nameIdentifier": "84738", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "acta41_01_08_t.pdf", "filesize": [{"value": "499.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 499900.0, "url": {"label": "acta41_01_08_t.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/20106/files/acta41_01_08_t.pdf"}, "version_id": "bd81304d-ab9c-45c3-a73b-f740720fb6d8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "subfragment-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "adult chicken gizzard myosin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "amino acid sequence", "subitem_subject_scheme": "Other"}, {"subitem_subject": "soform", "subitem_subject_scheme": "Other"}, {"subitem_subject": "smooth muscle", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Direct Sequence Analysis of a Cyanogen Bromide-Generated Peptide Corresponding to the Region of Subfragment-1 of Adult Chicken Gizzard Myosin Heavy Chain Predicted to Contain a Seven-Amino Acid Insertion", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Direct Sequence Analysis of a Cyanogen Bromide-Generated Peptide Corresponding to the Region of Subfragment-1 of Adult Chicken Gizzard Myosin Heavy Chain Predicted to Contain a Seven-Amino Acid Insertion"}]}, "item_type_id": "3", "owner": "2", "path": ["1389"], "permalink_uri": "http://hdl.handle.net/10069/16051", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-03-13"}, "publish_date": "2008-03-13", "publish_status": "0", "recid": "20106", "relation": {}, "relation_version_is_last": true, "title": ["Direct Sequence Analysis of a Cyanogen Bromide-Generated Peptide Corresponding to the Region of Subfragment-1 of Adult Chicken Gizzard Myosin Heavy Chain Predicted to Contain a Seven-Amino Acid Insertion"], "weko_shared_id": -1}
Direct Sequence Analysis of a Cyanogen Bromide-Generated Peptide Corresponding to the Region of Subfragment-1 of Adult Chicken Gizzard Myosin Heavy Chain Predicted to Contain a Seven-Amino Acid Insertion
http://hdl.handle.net/10069/16051
http://hdl.handle.net/10069/1605115253568-6033-41ab-bdbe-d4c59bf38b4b
名前 / ファイル | ライセンス | アクション |
---|---|---|
acta41_01_08_t.pdf (499.9 kB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-03-13 | |||||
タイトル | ||||||
タイトル | Direct Sequence Analysis of a Cyanogen Bromide-Generated Peptide Corresponding to the Region of Subfragment-1 of Adult Chicken Gizzard Myosin Heavy Chain Predicted to Contain a Seven-Amino Acid Insertion | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | subfragment-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adult chicken gizzard myosin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | amino acid sequence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | soform | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | smooth muscle | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Sakurai, Masaharu
× Sakurai, Masaharu× Watanabe, Bunji× Nagata, Shuichi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The amino acid sequence of a cyanogen bromide-generated peptide corresponding to the region of subfragment-1 of chicken gizzard myosin, predicted from the cDNA sequence to contain a seven-amino acid insertion, was determined directly. Subfragment-1 was prepared from adult chicken gizzard myosin by limited digestion with papain, and a 137-residue peptide was obtained by cleaving subfragment-1 with cyanogen bromide. Amino acid composition analysis and sequence analysis of the fragments generated from this peptide by formic acid or a-chymotrypsin revealed that the peptide corresponded to leucine-165 to methionine-301 of chicken gizzard myosin heavy chain previously deduced from the cDNA sequence by Yanagisawa, M. et al.. The predicted seven-amino acid insertion (Gln-Gly-Pro-Ser-Phe-Ser-Tyr), absent from other smooth muscle myosin,was thus confirmed to be present in chicken gizzard myosin. The amino acid sequence surrounding the insertion site of chicken gizzard myosin shows greater homology to the corresponding sequence of other known smooth muscle and non-muscle myosins than to those of chicken skeletal and cardiac muscle myosins. The insertion sequence may confer gizzard-specific functions to the gizzard myosin heavy chain, whereas the amino acid sequence surrounding the insertion site may be specific to smooth muscle. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 41, 号 1-2, p. 53-57, 発行日 1996-06-20 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00053-P00057 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1996, 41(1-2), p.53-57 |