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Mapping and Ordering Cosmid Clones Obtained from Human Chromosome Region 11 q 13.4-q25 by Fluorescence in situ Hybridization
http://hdl.handle.net/10069/16061
http://hdl.handle.net/10069/16061cfb5485b-f4db-4869-a179-a7ab0f661685
名前 / ファイル | ライセンス | アクション |
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acta41_03_05_t.pdf (799.1 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-03-13 | |||||
タイトル | ||||||
タイトル | Mapping and Ordering Cosmid Clones Obtained from Human Chromosome Region 11 q 13.4-q25 by Fluorescence in situ Hybridization | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Kato, Rumiko
× Kato, Rumiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A cosmid library was screened with 40 sequence-tagged sites (STSs) designed previously on the basis of DNA sequences of microclones that were generated by chromosome microdissection on two different human chromosomal regions, 11q14-q22 and 11q23-q25. Seventeen cosmid clones that corresponded to the STSs were successfully isolated. Single-color fluorescence in situ hybridization (FISH) using the 17 cosmids as probes, 11 were mapped to the expected regions of 11q. Then, the order of localization of the 7 cosmids (cG20, cG38, cG38R, cG50, cG51, cG75 and cR54) at the 11q21-q22.3 region were determined by two-color FISH on the extended prometaphase chromosomes, as cen-cG50-cG75- cG51-cG38-cG38R-cG20-cR54-tel. The result not only confirmed that microdissection is useful in the isolation of region-specific DNA clones, but also indicated that subsequent single- or two-color FISH is a valuable tool to map novel clones and marker DNAs, especially their order in the human genome. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 41, 号 3-4, p. 26-30, 発行日 1996-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00026-P00030 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1996, 41(3-4), p.26-30 |