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Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency
http://hdl.handle.net/10069/16871
http://hdl.handle.net/10069/1687145cc4c52-6f89-4085-bd8c-95af823fa337
名前 / ファイル | ライセンス | アクション |
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PPB46_452.pdf (288.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-05-07 | |||||
タイトル | ||||||
タイトル | Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hyoscyamus albus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hairy roots | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | iron deficiency | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | riboflavin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | citric acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | malic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proton-pump inhibitor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Abbreviations | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DCCD, N,N’-dicyclohexylcarbodiimide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | FAD, flavinadenindinucleotide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | FMN, flavinmononucleotide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | H6H, hyoscyamine 6β-hydroxylase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPLC, high-performance liquid chromatography | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MS, Murashige and Skoog. | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Higa, Ataru
× Higa, Ataru× Miyamoto, Erika× Rahman, Laiq ur× Kitamura, Yoshie |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Hyoscyamus albus hairy roots with/without an exogenous gene (11 clones) were established by inoculation of Agrobacterium rhizogenes. All clones cultured under iron deficient condition secreted riboflavin from root tips into the culture medium and the productivity depended on the number and size of root tips among the clones, although the addition of sucrose was essential for riboflavin production. A decline of pH was observed before riboflavin production and root development using either a root tip or propagated roots: propagated roots were employed for further work due to their lesser variation. Additions of proton-pump inhibitors, N,N’-dicyclohexylcarbodiimide (DCCD) at 100 and 10 μM and erythrosine B at 100 μM, suppressed the pH decline at 100 and 10 μM accompanied by inhibition of riboflavin secretion and root growth; at 10 μM of erythrosine B, pH decline occurred with a moderate delay, but both growth and riboflavin efflux were inhibited. Neither inhibition of the pH decline nor riboflavin production was observed at 1 μM. To examine the necessity of acidification and riboflavin secretion by the roots themselves, artificial pH reduction of culture medium with organic acids and the addition of exogenous riboflavin with/without pH reduction were performed. When hairy roots were cultured in iron-deficient medium acidified with citric acid (pH 4.0) or malic acid (pH 3.7), pH increased rapidly to around 5 overnight, following which riboflavin production and root growth occurred. Addition of riboflavin did not affect riboflavin secretion by the roots, but acidification with citric acid (pH 4.0) helped achiever greater riboflavin production and earlier pH elevation. These results indicate that riboflavin efflux does not directly connected to active pH reduction, and more significantly active riboflavin secretion occurs by internal requirement in H. albus hairy roots under iron deficiency. | |||||
書誌情報 |
Plant Physiology and Biochemistry 巻 46, 号 4, p. 452-460, 発行日 2008-04-04 |
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出版者 | ||||||
出版者 | Elsevier Masson SAS. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09819428 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11537248 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18367404 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.plaphy.2008.01.004 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2008 Elsevier Masson SAS. All rights | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant Physiology and Biochemistry 46(4), 452-460;2008 |