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Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells
http://hdl.handle.net/10069/22014
http://hdl.handle.net/10069/22014166c6464-4b3f-4a85-9f4f-c3e66f411443
名前 / ファイル | ライセンス | アクション |
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CanSci99_1147.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-07-01 | |||||
タイトル | ||||||
タイトル | Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Anaplastic thyroid cancer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | miRNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | miR-17-92 cluster | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | miR-17-3p | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | miR-17-5p | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | miR-19a | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Takakura, Shu
× Takakura, Shu× Mitsutake, Norisato× Nakashima, Masahiro× Namba, Hiroyuki× Saenko, Vladimir A.× Rogounovitch, Tatiana I.× Nakazawa, Yuka× Hayashi, Tomayoshi× Ohtsuru, Akira× Yamashita, Shunichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Micro RNAs (miRNAs) are non-coding small RNAs and constitute a novel class of negative gene regulators that are found in both plants and animals. Several miRNAs play crucial roles in cancer cell growth. To identify miRNAs specifically deregulated in anaplastic thyroid cancer (ATC) cells, we performed a comprehensive analysis of miRNA expressions in ARO cells and primary thyrocytes using miRNA microarrays. MiRNAs in a miR-17-92 cluster were overexpressed in ARO cells. We confirmed the overexpression of those miRNAs by Northern blot analysis in ARO and FRO cells. In 3 of 6 clinical ATC samples, miR-17-3p and miR-17-5p were robustly overexpressed in cancer lesions compared to adjacent normal tissue. To investigate the functional role of these miRNAs in ATC cells, ARO and FRO cells were transfected with miRNA inhibitors, antisense oligonucleotides containing locked nucleic acids. Suppression of miR-17-3p caused complete growth arrest, presumably due to caspase activation resulting in apoptosis. MiR-17-5p or miR-19a inhibitor also induced strong growth reduction, but only miR-17-5p inhibitor led to cellular senescence. On the other hand, miR-18a inhibitor only moderately attenuated the cell growth. Thus, we have clarified functional differences among the members of the cluster in ATC cells. In conclusion, these findings suggest that the miR-17-92 cluster plays an important role in certain types of ATCs and could be a novel target for ATC treatment. | |||||
書誌情報 |
Cancer Science 巻 99, 号 6, p. 1147-1154, 発行日 2008-06 |
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出版者 | ||||||
出版者 | Blackwell Publishing | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13479032 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1349-7006 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11808050 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18429962 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/j.1349-7006.2008.00800.x | |||||
権利 | ||||||
権利情報 | c 2008 Japanese Cancer Association | |||||
権利 | ||||||
権利情報 | The definitive version is available at www.blackwell-synergy.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cancer Science, 99(6), pp.1147-1154; 2008 |