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Spleen-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Spleen Surface in Mice
http://hdl.handle.net/10069/15099
http://hdl.handle.net/10069/150999fd762fd-f787-435a-8201-e4d6110d4711
名前 / ファイル | ライセンス | アクション |
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bpb_30_941.pdf (189.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-03-05 | |||||
タイトル | ||||||
タイトル | Spleen-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Spleen Surface in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | spleen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transfection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mouse | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | luciferase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakamura, Junzo
× Nakamura, Junzo× Fumoto, Shintaro× Kawanami, Rie× Kodama, Yukinobu× Nishi, Junya× Nakashima, Mikiro× Sasaki, Hitoshi× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of present study was to examine spleen-selective gene transfer following the administration of naked plasmid DNA (pDNA) onto the spleen surface in mice. Gene expression in the spleen and other tissues was evaluated based on firefly luciferase activity. Six hours after spleen surface instillation of naked pDNA, high gene expression in the spleen was observed. On the contrary, intravenous and intraperitoneal administration of naked pDNA resulted in no detectable gene expression. After instilling naked pDNA onto the spleen surface, gene expression in the spleen was significantly higher than those in other tissues. Six hours after instillation of naked pDNA onto the spleen surface, gene expression in the spleen reached the peak value, and thereafter decreased gradually. By utilizing a glass-made diffusion cell that is able to limit the contact dimension between the spleen surface and naked pDNA solution administered, site-specific gene expression in the spleen was found. This novel gene transfer method is expected to be a safe and effective strategy for DNA vaccine against serious infectious diseases and cancers. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 30, 号 5, p. 941-945, 発行日 2007-05 |
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出版者 | ||||||
出版者 | The Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00602100 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 17473439 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.30.941 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/bpb.30.941 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin v.30(5) p.941-945, 2007 |