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Kinetic Changes of DNA and RNA Contents in Activated Lymphocytes upon Lectin Stimulation and Operative Insult
http://hdl.handle.net/10069/15681
http://hdl.handle.net/10069/156811db1d918-ecb7-490e-9fe5-52db20b1684f
名前 / ファイル | ライセンス | アクション |
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acta30_01_21_t.pdf (973.7 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-03-10 | |||||
タイトル | ||||||
タイトル | Kinetic Changes of DNA and RNA Contents in Activated Lymphocytes upon Lectin Stimulation and Operative Insult | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Ishibashi, Tsunehisa
× Ishibashi, Tsunehisa |
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著者別名 | ||||||
姓名 | 石橋, 経久 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The cellular DNA and RNA contents of the lymphocytes were assessed by using a Fluorescence Activating Cell Sortor System (FACS-IV)to evaluate the lymphocyte response to the lectin stimulation or the operative insult . 1) The DNA and RNA contents in the lymphocytes stimulated by the lectins such as phytohemagglutinin (PHA), Concanavalin A (Con A) and Pokeweed mitogen (PWM) were measured in vitro by using the technique of FACS-IV in comparision with those by the uptakes of 3H-thymidine dR and Uridine R . The DNA and RNA syntheses began to start at the first 6 hours of stimulation by the lectins in accordance with an appearance of the lymphocyte in early S phase and it was apparent that mitosis occurred for a period between 36 and 48 hours . It is to be further emphasized that DNA syntheses in the lectin-stimulated lymphocytes were initiated with the passage of 6 hours and remarkably increasing as those at 12 and 24 hr . 2) Postoperative states were evaluated from the standpoint of change in the cellular RNA contents in the monocytes. An increase in the RNA content was noticeable on day 1 to day 7 following surgery and these changes were associated with postoperatively serious conditions such as either grave complication or major operative invasion . In conclusion, the assessment of the cellular RNA content in the monocytes plays an important role in identifying postoperative state from serious condition on the course of postoperative period. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 30, 号 1-3, p. 225-237, 発行日 1985-10-25 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00225-P00237 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1985, 30(1-3), p.225-237 |