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Receptor Autoradiographic Analysis of Muscarinic Receptors in the Rat Atrioventricular Node
http://hdl.handle.net/10069/15855
http://hdl.handle.net/10069/15855e7c8538b-8450-4e7c-ae7d-c2a85f0e328d
名前 / ファイル | ライセンス | アクション |
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acta35_01_55_t.pdf (554.1 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-03-13 | |||||
タイトル | ||||||
タイトル | Receptor Autoradiographic Analysis of Muscarinic Receptors in the Rat Atrioventricular Node | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Yamasaki, Hidefumi
× Yamasaki, Hidefumi× Niwa, Masami× Shigematsu, Kazuto× Seto, Shinji× Oku, Yasuhiko× Ozaki, Masayori× Hashiba, Kunitake |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We carried out investigations on muscarinic acetylcholine receptors (m-AChR) in the rat heart, including the atrioventricular (AV) node. The related tissue sections were incubated with 3H-quinuclidinyl benzilate (3H-QNB), then autoradiography and an image analysis coupled with computer-assisted microdensitometry were done. A single type of specific and high affinity binding sites of 3H-QNB was found to be highly concentrated in the AV node, the maximum binding capacity (Bmax) being 1.4 pmol/mg protein and with a dissociation constant (Kd) of 0.5 nM. The density and affinity of the binding to the AV node were the highest, when compared with findings in the atrium (interatrial septum) and ventricle (interventricular septum). The binding was competitively displaced by AF-DX 116, a selective antagonist for the M2 AChR subtype, with a high affinity, whereas pirenzepine, an antagonist for the M1 AChR subtype was much less potent in displacing the binding. Therefore, vagal-cholinergic stimulation presumably plays a significant role in functions of the rat AV node, probably by interacting with the specific, high affinity M2 AChR subtype. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 35, 号 1-4, p. 298-303, 発行日 1990-12-14 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00298-P00303 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1990, 35(1-4), p.298-303 |