WEKO3
アイテム
{"_buckets": {"deposit": "84bb59b2-4f17-4050-a6bd-d6434eb154cc"}, "_deposit": {"created_by": 2, "id": "22027", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "22027"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00022027", "sets": ["1563"]}, "author_link": ["94009", "94007", "94006", "94008", "94010", "94005"], "item_3_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1993-12-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2-4", "bibliographicPageEnd": "178", "bibliographicPageStart": "175", "bibliographicVolumeNumber": "38", "bibliographic_titles": [{"bibliographic_title": "Acta medica Nagasakiensia"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We determined the nucleotide sequence of a region of the 12S ribosomal RNA (rRNA) gene in the mitochondrial DNA (mtDNA) of the sea snake, Laticauda semifasciata, using the polymerase chain reaction (PCR). We synthesized oligonucleotide primers according to the nucleotide sequence of human mt DNA 12S rRNA gene and found that the target sequence (386bp) of the sea snake mtDNA could be amplified with these primers. The nucleotide sequence of the amplified region of the sea snake mt DNA was determined on six separate plasmid clones for each individual snake DNA and matched completely among the DNA samples of three sea snakes. The sequence homology in the region of the mtDNA 12S rRNA gene between L. semifasciata and human is 69.1%.", "subitem_description_type": "Abstract"}]}, "item_3_description_64": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Acta medica Nagasakiensia. 1993, 38(2-4), p.175-178", "subitem_description_type": "Other"}]}, "item_3_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508430", "subitem_source_identifier_type": "NCID"}]}, "item_3_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00016055", "subitem_source_identifier_type": "ISSN"}]}, "item_3_text_62": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "P00175-P00178"}]}, "item_3_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Eguchi, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "94005", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eguchi, Yukinori"}], "nameIdentifiers": [{"nameIdentifier": "94006", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oshiro, Minoru"}], "nameIdentifiers": [{"nameIdentifier": "94007", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Asato, Tsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "94008", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takei, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "94009", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakashima, Yasutsugu"}], "nameIdentifiers": [{"nameIdentifier": "94010", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "acta38_02_29_t.pdf", "filesize": [{"value": "453.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 453200.0, "url": {"label": "acta38_02_29_t.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/22027/files/acta38_02_29_t.pdf"}, "version_id": "9918dae3-13fb-4131-98dd-b869cd9c98e6"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "DNA Amplification and Nucleotide Sequence Determination of a Region of Mitochondrial DNA in the Sea Snake, Laticauda Semifasciata", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "DNA Amplification and Nucleotide Sequence Determination of a Region of Mitochondrial DNA in the Sea Snake, Laticauda Semifasciata"}]}, "item_type_id": "3", "owner": "2", "path": ["1563"], "permalink_uri": "http://hdl.handle.net/10069/15932", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-03-13"}, "publish_date": "2008-03-13", "publish_status": "0", "recid": "22027", "relation": {}, "relation_version_is_last": true, "title": ["DNA Amplification and Nucleotide Sequence Determination of a Region of Mitochondrial DNA in the Sea Snake, Laticauda Semifasciata"], "weko_shared_id": -1}
DNA Amplification and Nucleotide Sequence Determination of a Region of Mitochondrial DNA in the Sea Snake, Laticauda Semifasciata
http://hdl.handle.net/10069/15932
http://hdl.handle.net/10069/15932bfbe811c-5681-454a-bd59-138948025676
名前 / ファイル | ライセンス | アクション |
---|---|---|
acta38_02_29_t.pdf (453.2 kB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-03-13 | |||||
タイトル | ||||||
タイトル | DNA Amplification and Nucleotide Sequence Determination of a Region of Mitochondrial DNA in the Sea Snake, Laticauda Semifasciata | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Eguchi, Tomoko
× Eguchi, Tomoko× Eguchi, Yukinori× Oshiro, Minoru× Asato, Tsuyoshi× Takei, Hiroshi× Nakashima, Yasutsugu |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We determined the nucleotide sequence of a region of the 12S ribosomal RNA (rRNA) gene in the mitochondrial DNA (mtDNA) of the sea snake, Laticauda semifasciata, using the polymerase chain reaction (PCR). We synthesized oligonucleotide primers according to the nucleotide sequence of human mt DNA 12S rRNA gene and found that the target sequence (386bp) of the sea snake mtDNA could be amplified with these primers. The nucleotide sequence of the amplified region of the sea snake mt DNA was determined on six separate plasmid clones for each individual snake DNA and matched completely among the DNA samples of three sea snakes. The sequence homology in the region of the mtDNA 12S rRNA gene between L. semifasciata and human is 69.1%. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 38, 号 2-4, p. 175-178, 発行日 1993-12-25 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00175-P00178 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1993, 38(2-4), p.175-178 |