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The Role of Phosphorylation in IR-induced p53 Stabilization and Activation
http://hdl.handle.net/10069/9312
http://hdl.handle.net/10069/93121964fc3f-0e7c-4032-99c5-fe6aeb16f260
名前 / ファイル | ライセンス | アクション |
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acta50_s1_51.pdf (463.9 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-11-02 | |||||
タイトル | ||||||
タイトル | The Role of Phosphorylation in IR-induced p53 Stabilization and Activation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | p53 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phosphorylation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Stabilization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Activation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Yamauchi, Motohiro
× Yamauchi, Motohiro× Suzuki, Keiji× Kodama, Seiji× Watanabe, Masami |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It is well established that tumor suppressor p53 protein is stabilized and activated as a sequence-specific transcriptional factor in response to genotoxic stresses, including ionizing radiation (IR). Previous studies demonstrated that p53 is phosphorylated at N-terminal serines and a threonine after IR. Here, we examined the role of the phosphorylation, especially at Ser15, Thr18, and Ser20, in IR-induced p53 stabilization and activation, using alanine-substituted p53 at phosphorylation site (s). In the present study, we examined p53 stabilization and activation in two different cell lines : stabilization in HT1080-derived clone in which endogenous wild-type p53 was confirmed to be normally stabilized after IR; activation in p53-null H1299-derived clone in which ectopically-expressed p53 levels did not change before and after IR, because the increase in p53 levels affects transactivation by p53. In Western blot analysis and immunofluorescence staining, alanine-substituted p53 at Ser15, Thr18, and/or Ser20 was stabilized in the nucleus comparably with wild-type p53 2 h after 4 Gy of X-rays. However, Ser15-alanine-substituted p53 did not induce the expression of p21 protein, one of the p53-targeted gene products, after IR. These results indicate that phosphorylation at Ser15, Thr18, and Ser20 is not required for IR-induced p53 stabilization, however, Ser15-phosphorylation plays a role in IR-induced p21 induction by p53. | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 50, 号 supl.1, p. 51-54, 発行日 2005-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://joi.jlc.jst.go.jp/JST.JSTAGE/amn/50.S51 | |||||
sortkey | ||||||
P00051-P00054 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 2005, 50(supl.1), p.51-54 |