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Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by γ-Proteobacterium and Its Multiple Bioactivities
http://hdl.handle.net/10069/8333
http://hdl.handle.net/10069/833335ae7f1d-49e1-4516-a457-1dc62c923ec1
名前 / ファイル | ライセンス | アクション |
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BPBul28_2289.pdf (593.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-05 | |||||
タイトル | ||||||
タイトル | Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by γ-Proteobacterium and Its Multiple Bioactivities | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | prodigiosin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | apoptosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | superoxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | U937 cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γ-proteobacterium | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nakashima, Takuji
× Nakashima, Takuji× Tamura, Tadashi× Kurachi, Maki× Yamaguchi, Kenichi× Oda, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recently we discovered a bacterial strain (MS-02-063) that produces large amounts of red pigment (PG-L-1). Among the cell lines tested, U937 cells showed the highest susceptibility to PG-L-1 toxicity. PG-L-1 induced typical apoptotic nuclear morphological changes, and single cell gel electrophoresis revealed that PG-L-1 caused DNA fragmentation in U937 cells. In PG-L-1 treated U937 cells, the acidic compartment such as lysosomes disappeared, suggesting that PG-L-1-induced disorder of intracellular pH compartmentalization might trigger apoptotic signal. Since p38 MAP kinase inhibitor specifically prevented the PG-L-1 mediated cell death, p38 MAP kinase may be involved in the cytotoxic mechanism. In fact, immunoblot analysis of p38 MAP kinase revealed that phosphorylation of p38 MAP kinase occurred in PG-L-1-treated U937 cells. In addition to the activity to induce apoptotic cell death as reported in several PG family members, our chemiluminescence analysis suggested that PG-L-1 inhibited superoxide generation by 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated U937 cells in a dose-dependent manner. Since PG-L-1 had no effect on the chemiluminescence response caused by xanthine oxidase/hypoxanthine system, PG-L-1 acts on the enzyme system responsible for O_2- generation rather than direct scavenging toward O_2-. Our results suggest that PG-L-1 causes multiple biochemical effects on the target cells such as increase in pH in acidic intracellular compartment, activation of p38 MAP kinase, inhibition of O_2- generation, and eventually induces apoptotic cell death. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 28, 号 12, p. 2289-2295, 発行日 2005-12 |
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出版者別言語 | ||||||
Pharmaceutical Society of Japan. | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 16327167 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.28.2289 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/bpb.28.2289 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin v.28(12) p.2289-2295, 2005 |