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Partial Hepatectomy Enhances Polyethylenimine-Mediated Plasmid DNA Delivery
http://hdl.handle.net/10069/8340
http://hdl.handle.net/10069/8340a0867c51-9366-451a-b2a3-f87f660cdce9
名前 / ファイル | ライセンス | アクション |
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BPBul29_1712.pdf (177.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-12 | |||||
タイトル | ||||||
タイトル | Partial Hepatectomy Enhances Polyethylenimine-Mediated Plasmid DNA Delivery | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | non-viral vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polyethylenimine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | partial hepatectomy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tada, Yuki
× Tada, Yuki× Kitahara, Takashi× Yoshioka, Takashi× Nakamura, Tadahiro× Ichikawa, Nobuhiro× Nakashima, Mikiro× Nishida, Koyo× Nakamura, Junzo× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Polyethylenimine (PEI) is widely used for non-viral transfection in vitro and in vivo. Hepatectomy is an interesting and considerable factor modifying PEI-mediated gene expression. We investigated the gene expression in mice over time following partial hepatectomy after an intravenous injection of PEI/plasmid DNA (pDNA) complex. pDNA encoding firefly luciferase was used as the model reporter gene. The hepatectomized liver was rapidly regenerated until 72 h. After 168 h, the liver weight of hepatectomized mice was similar to that of control mice. Slight liver function impairment was only observed at 1?24 h after hepatectomy in alanine aminotransferase and aspartate aminotransferase levels. Luciferase activity in the liver of partial hepatectomized mice at 48 h after partial hepatectomy increased by 70 times compared with that of control mice; however, luciferase activities did not significantly differ between hepatectomized mice and control mice in the spleen, lung, kidney, and heart. Among the lobes, luciferase activity by gram of tissue was not significantly different, indicating that gene expression enhancement by partial hepatectomy occurred equally throughout the liver. In conclusion, our findings demonstrate that liver resection is an influencing factor on PEI-mediated gene delivery in mice. These results indicate the necessity of considering cell division in PEI-mediated pDNA delivery. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 29, 号 8, p. 1712-1716, 発行日 2006-08 |
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出版者 | ||||||
出版者 | Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 16880630 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.29.1712 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/bpb.29.1712 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin v.29(8) p.1712-1716, 2006 |