WEKO3
アイテム
{"_buckets": {"deposit": "e22203e0-b824-4ae6-83de-da7e8d44c390"}, "_deposit": {"created_by": 2, "id": "2380", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "2380"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00002380", "sets": ["28"]}, "author_link": ["11076", "11078", "11079", "11080", "11075", "11073", "11077", "11081", "11074"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageStart": "e00277-17", "bibliographicVolumeNumber": "85", "bibliographic_titles": [{"bibliographic_title": "Infection and Immunity"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Severe periodontitis is known to aggravate diabetes mellitus, though molecular events related to that link have not been fully elucidated. Porphyromonas gingivalis, a major pathogen of periodontitis, expresses dipeptidyl peptidase 4 (DPP4), which is involved in regulation of blood glucose levels by cleaving incretins in humans. We examined the enzymatic characteristics of DPP4 from P. gingivalis as well as two other periodontopathic bacteria, Tannerella forsythia and Prevotella intermedia, and determined whether it is capable of regulating blood glucose levels. Cell-associated DPP4 activity was found in those microorganisms, which was effectively suppressed by inhibitors of human DPP4, and molecules sized 73 kDa in P. gingivalis, and 71 kDa in T. forsythia and P. intermedia were immunologically detected. The kcat/Km values of recombinant DPP4s ranged from 721 ± 55 to 1283 ± 23 μM-1sec-1 toward Gly-Pro-4-methylcoumaryl-7-amide (MCA), while those were much lower for His-Ala-MCA. MALDI-TOF MS analysis showed the His/Tyr-Ala dipeptide release from the N-termini of incretins, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide, respectively, with the action of microbial DPP4. Moreover, intravenous injection of DPP4 into mice decreased plasma active GLP-1 and insulin levels, accompanied by a substantial elevation in blood glucose over the control after oral glucose administration. These results are the first to show that periodontopathic bacterial DPP4 is capable of modulating blood glucose levels the same as mammalian DPP4, thus the incidence of periodontopathic bacteremia may exacerbate diabetes mellitus via molecular events of bacterial DPP4 activities.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Infection and Immunity, 85(9), e00277-17; 2017", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Microbiology"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1128/IAI.00277-17", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2017 American Society for Microbiology."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00199567", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "10985522", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ohara-Nemoto, Yuko"}], "nameIdentifiers": [{"nameIdentifier": "11073", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakasato, Manami"}], "nameIdentifiers": [{"nameIdentifier": "11074", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimoyama, Yu"}], "nameIdentifiers": [{"nameIdentifier": "11075", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Baba, Tomomi T."}], "nameIdentifiers": [{"nameIdentifier": "11076", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayakawa, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "11077", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono, Toshio"}], "nameIdentifiers": [{"nameIdentifier": "11078", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yaegashi, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "11079", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kimura, Shigenobu"}], "nameIdentifiers": [{"nameIdentifier": "11080", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nemoto, Takayuki K."}], "nameIdentifiers": [{"nameIdentifier": "11081", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "InfImm85_00277-17.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "InfImm85_00277-17.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/2380/files/InfImm85_00277-17.pdf"}, "version_id": "feda4ae1-32ce-49a3-9a3d-45958c93735c"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "blood glucose", "subitem_subject_scheme": "Other"}, {"subitem_subject": "diabetes mellitus", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GIP", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GLP-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "incretins", "subitem_subject_scheme": "Other"}, {"subitem_subject": "periodontitis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Porphyromonas gingivalis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prevotella intermedia", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tannerella forsythia", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Degradation of Incretins and Modulation of Blood Glucose Levels by Periodontopathic Bacterial Dipeptidyl Peptidase 4", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Degradation of Incretins and Modulation of Blood Glucose Levels by Periodontopathic Bacterial Dipeptidyl Peptidase 4"}]}, "item_type_id": "2", "owner": "2", "path": ["28"], "permalink_uri": "http://hdl.handle.net/10069/37736", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-04-01"}, "publish_date": "2018-04-01", "publish_status": "0", "recid": "2380", "relation": {}, "relation_version_is_last": true, "title": ["Degradation of Incretins and Modulation of Blood Glucose Levels by Periodontopathic Bacterial Dipeptidyl Peptidase 4"], "weko_shared_id": -1}
Degradation of Incretins and Modulation of Blood Glucose Levels by Periodontopathic Bacterial Dipeptidyl Peptidase 4
http://hdl.handle.net/10069/37736
http://hdl.handle.net/10069/37736025043ac-720b-4f38-a6b8-c4d2b62c2521
名前 / ファイル | ライセンス | アクション |
---|---|---|
InfImm85_00277-17.pdf (1.1 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2018-04-01 | |||||
タイトル | ||||||
タイトル | Degradation of Incretins and Modulation of Blood Glucose Levels by Periodontopathic Bacterial Dipeptidyl Peptidase 4 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | blood glucose | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | diabetes mellitus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GIP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GLP-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | incretins | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | periodontitis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Prevotella intermedia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tannerella forsythia | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ohara-Nemoto, Yuko
× Ohara-Nemoto, Yuko× Nakasato, Manami× Shimoyama, Yu× Baba, Tomomi T.× Kobayakawa, Takeshi× Ono, Toshio× Yaegashi, Takashi× Kimura, Shigenobu× Nemoto, Takayuki K. |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Severe periodontitis is known to aggravate diabetes mellitus, though molecular events related to that link have not been fully elucidated. Porphyromonas gingivalis, a major pathogen of periodontitis, expresses dipeptidyl peptidase 4 (DPP4), which is involved in regulation of blood glucose levels by cleaving incretins in humans. We examined the enzymatic characteristics of DPP4 from P. gingivalis as well as two other periodontopathic bacteria, Tannerella forsythia and Prevotella intermedia, and determined whether it is capable of regulating blood glucose levels. Cell-associated DPP4 activity was found in those microorganisms, which was effectively suppressed by inhibitors of human DPP4, and molecules sized 73 kDa in P. gingivalis, and 71 kDa in T. forsythia and P. intermedia were immunologically detected. The kcat/Km values of recombinant DPP4s ranged from 721 ± 55 to 1283 ± 23 μM-1sec-1 toward Gly-Pro-4-methylcoumaryl-7-amide (MCA), while those were much lower for His-Ala-MCA. MALDI-TOF MS analysis showed the His/Tyr-Ala dipeptide release from the N-termini of incretins, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide, respectively, with the action of microbial DPP4. Moreover, intravenous injection of DPP4 into mice decreased plasma active GLP-1 and insulin levels, accompanied by a substantial elevation in blood glucose over the control after oral glucose administration. These results are the first to show that periodontopathic bacterial DPP4 is capable of modulating blood glucose levels the same as mammalian DPP4, thus the incidence of periodontopathic bacteremia may exacerbate diabetes mellitus via molecular events of bacterial DPP4 activities. | |||||
書誌情報 |
Infection and Immunity 巻 85, 号 9, p. e00277-17, 発行日 2017-09 |
|||||
出版者 | ||||||
出版者 | American Society for Microbiology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00199567 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10985522 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1128/IAI.00277-17 | |||||
権利 | ||||||
権利情報 | c 2017 American Society for Microbiology. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Infection and Immunity, 85(9), e00277-17; 2017 |