WEKO3
アイテム
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同時感染したHEK細胞を用いたアデアウイルス5型のプラーク法
http://hdl.handle.net/10069/4188
http://hdl.handle.net/10069/418871d92ee0-b7c6-4d0b-9a13-ba0740ddf965
名前 / ファイル | ライセンス | アクション |
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tm17_03_05_t.pdf (609.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | 同時感染したHEK細胞を用いたアデアウイルス5型のプラーク法 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
上田, 芳秋
× 上田, 芳秋× 明石, 光伸× 林, 薫 |
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著者別名 | ||||||
姓名 | Ueda, Yoshiaki | |||||
著者別名 | ||||||
姓名 | Akashi, Mitsunobu | |||||
著者別名 | ||||||
姓名 | Hayashi, Kaoru | |||||
その他のタイトル | ||||||
その他のタイトル | Plaque Assay Method for Adenovirus Type 5 with the Culture of HEK Cells Synchronously Infected with the Virus. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 適宜に階段稀釈した抗血清とX×10^4 PFUのウイルスを混じ室温1時間放置した後,直ちに1:100まで稀釈する.この稀釈液と等量の維持に浮游させた2~3×10^6/mlのHEK細胞とを混和し,37℃30分間軽く振盪する.径5cmのシヤレーに1mlずつ入れ,細胞をガラス面に拡げ,0.5%仔牛血清,0.75%カルボオキシメチールセルローズを含んだ維持液を加え蔽う.5日ないし6日目に細胞をギムザ液で染色し,プラークを算える.以上が抗血清の中和抗体の測定法である.抗血清と抗原(分画その他)を加え充分反応させた後,更らにX×10^4PFUのウイルスを追加し室温60分放置し,以後の手順は上記の方法に従う,以上が抗原による抗血清中の抗体のブロック能を知る方法である.以上の方法を用いて,アデノウイルス5型の抗血清に対するA抗原,C抗原及びP抗原のブロック能を検査した.抗血清が完全にブロックされたのはA抗原によってのみであり, C抗原及びP抗原は抗血清のブロック能を有しないことを知った. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The mixture of antiserum and antigen at each adequate dilution was added the seed virus contained X×10^4 PFU per ml. The mixture was diluted upto the concentration contained 50 to 100 plaques per ml per a dish and 2 ml of the last dilution was made. The last dilution of the mixture was added equal volume of HEK cell suspension contained 2 or 3×10^6 cells per ml and shaked at 37℃ for 20 or 30 minutes. One ml of these mixture of antiserum, antigen and seed virus was plated into a dish, and 4 ml of maintainance medium contained 0.5% calf serum and 0.75% carboxymethylcellulose was added and spread over in a dish. After incubation for 5 or 6 days, the cell sheet was fixed with 10% formaldehyde saline solution and washed and stained with Gimsa solution. The plaques formed in this method were clear and easy to count. The activity of antigen A, C and P of adenovirus type 5 for the blocking antibody against purified adenovirus type 5 was studied with the application of this plaque assay method. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 17, 号 3, p. 151-158, 発行日 1976-01-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00151-P00158 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 17(3). p151-158, 1976 |