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HBs抗原染色の改良法と染色機構
http://hdl.handle.net/10069/4286
http://hdl.handle.net/10069/42865445e26a-248c-44d9-86ae-6abbc18afedb
名前 / ファイル | ライセンス | アクション |
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tm22_03_05_t.pdf (2.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | HBs抗原染色の改良法と染色機構 | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
千馬, 正敬
× 千馬, 正敬× 山下, 裕人× 板倉, 英世 |
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著者別名 | ||||||
姓名 | Senba, Masachika | |||||
著者別名 | ||||||
姓名 | Yamashita, Hiroto | |||||
著者別名 | ||||||
姓名 | Itakura, Hideyo | |||||
その他のタイトル | ||||||
その他のタイトル | Modified Staining Method for Hepatitis B Surface Antigen (HBs Ag) and Its Mechanism | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | HBs Ag in the hepatocytes have been stained by orcein methods. However, not only HBs Ag but also degenerative and necrotic cytoplasm of hepatocytes are occasionally stained by the method, that makes it difficult to identify HBs Ag. Moreover, orcein dyes have different staining properties from lots to lots, that causes difficulty in obtaining a stable result. Therefore, we tried to modify the orcein method and found that the procedure using a sensitizer (ferric ammonium sulfate or uranium nitrate) after oxidizing solution gave stable and satisfactory results. By this method, in the hepatocytes, only HBs Ag are stained positively. The mechanism of function of the sensitizer is not clear, but we considered that Fe or U ions occupy small gaps in the protain molecular structure in the cells before orcein molecules occupy these gaps and that the method does not give a false positive result. It is also suggested that various HBs-staining-dyes can react with SO_3H groups which are formed by the oxidization of SS groups of proteins. We have developed a new staining method for HBs Ag, using Resorcin-fuchsin. The results of our method were also discussed. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 22, 号 3, p. 181-187, 発行日 1980-10-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00181-P00187 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 22(3). p181-187, 1980 |