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日本脳炎ウイルスに対する免疲酵素測定法(ELISA). III.ブタ血清中の抗体価の測定
http://hdl.handle.net/10069/4326
http://hdl.handle.net/10069/43262cf70836-38c4-4052-a172-f9ea0e5bdf4e
名前 / ファイル | ライセンス | アクション |
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tm24_02_05_t.pdf (1.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | 日本脳炎ウイルスに対する免疲酵素測定法(ELISA). III.ブタ血清中の抗体価の測定 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
分藤, 桂子
× 分藤, 桂子× 森田, 公一× 五十嵐, 章 |
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著者別名 | ||||||
姓名 | Bundo, Keiko | |||||
著者別名 | ||||||
姓名 | Morita, Koichi | |||||
著者別名 | ||||||
姓名 | Igarashi, Akira | |||||
その他のタイトル | ||||||
その他のタイトル | Enzyme-linked Immunosorbent Assay (ELISA) on Japanese Encephalitis Virus. III. Assay on Antibody Titers in Swine Sera | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1681年の長崎県下のと場ブタ血清330検体を用い,日本脳炎(JE)ウイルスに対する抗体価を,免疫酵素抗体法(ELSA)の間接法により測定した. ELISAによる抗JE免疫グロブリン抗体価の検出と血球凝集抑制法(HI)によるものは,ほとんど一致した.抗JE抗体のIgMクラスは, 2メルカプトエタノール(2ME)処理によるHI法で, 33検体が検出されたのに比べて, ELISAでは97検体が検出された. 97検体中, 65検体はELISAでのみIgM抗体が認められたが,一方, 2ME感受性HI抗体が証明され, IgM-ELISA抗体が陰性のものが1検体あった. IgM抗体陽性で2-ME感受性HI抗体が陰性の検体の大部分は,流行の末期の高い抗体価の認められたものであった. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Antibody titers against Japanese encephalitis (JE) virus were measured by indirect enzyme-linked immunosorbent assay (ELISA) using 330 sera from slaughtered swines in Nagasaki, 1981. Detection of anti-JE immunoglobulin (Ig) antibodies by the ELISA almost paralleled to that by the hemagglutination-inhibition (HI) and the titer by the ELISA was approximately 10 times higher than that by the HI. Presence of IgM class of anti-JE antibodies was detected in 97 specimens by the ELISA in contrast to 33 by the HI test using 2-mercaptoethanol (2ME) treatment. Six+y five of the 97 specimens were shown to possess IgM antibodies only by the ELISA, while there was a single specimen with demonstrable 2ME-sensitive HI antibodies and negative IgM-ELISA. Most of the specimens with positive IgM-ELISA and negative 2ME-sensitive HI antibodies were those with high-titered Ig antibodies found late in the epidemic season. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 24, 号 2, p. 87-102, 発行日 1982-06-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00087-P00102 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 24(2). p87-102, 1982 |