WEKO3
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CF11とウイルスの混合液をニトロセルロース膜で濾過した場合,膜に含まれるウイルスの37℃での耐熱性は1~2週間増大しているように見えたが,濾過直後の膜からウイルスは効率良く回収されなかった.", "subitem_description_type": "Abstract"}, {"subitem_description": "Infectivity of highly temperature-sensitive and attenuated poliovirus type 3 vaccine strain, Leon 12a_1b, at 35-37℃ was stabilized by 15-20% sucrose in the medium with 1 M MgCl_2. On the other hand, the virus stability was reduced by 10% polyethylene glycol (PEG, Mol. Wt. 6000) in the medium without MgCl_2, especially when the virus-PEG mixtures were incubated after freeze-drying. Fibrous cellulose CF11 apparently adsorbed majority of the virus and did not change its inactivation rate, when virus-cellulose mixtures were centrifuged and the precipitates were incubated at 37℃. 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庶糖と塩化マグネシウムによる3型ポリオ生ワクチンの耐熱性の増大
http://hdl.handle.net/10069/4516
http://hdl.handle.net/10069/4516eb39e713-f379-4045-b879-e2af2cf24e12
名前 / ファイル | ライセンス | アクション |
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tm30_02_02_t.pdf (763.1 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | 庶糖と塩化マグネシウムによる3型ポリオ生ワクチンの耐熱性の増大 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
SRIVASTAVA, Ashok Kumar
× SRIVASTAVA, Ashok Kumar× MATYASOVA, Irena× KOZA, Jiri |
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著者別名 | ||||||
姓名 | Srivastava, Ashok Kumar | |||||
著者別名 | ||||||
姓名 | Matyasova, Irena | |||||
著者別名 | ||||||
姓名 | Koza, Jiri | |||||
その他のタイトル | ||||||
その他のタイトル | Thermal Stabilization of Poliovirus Type 3 Live Vaccine Strain by Sucrose in the Presence of Magnesium Chloride | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 熱に極めて不安定な3型ポリオウイルス生ワクチンLeon 12a_1b株の耐熱性を庶糖と塩化マグネシウムによって増大することができた.分子量6,000のポリオエチレングリコール(PEG)はこのウイルスの耐熱性を低下し,その効果はPEGとウイルスの混合液を凍結乾燥した場合に殊に顕著であった.このウイルスは繊維性セルロースCF11に吸着され, CF11とウイルスの混合液を遠心して得られた沈澱中のウイルスの37℃での耐熱性はCF11を加えなかった対照と変らなかった. CF11とウイルスの混合液をニトロセルロース膜で濾過した場合,膜に含まれるウイルスの37℃での耐熱性は1~2週間増大しているように見えたが,濾過直後の膜からウイルスは効率良く回収されなかった. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Infectivity of highly temperature-sensitive and attenuated poliovirus type 3 vaccine strain, Leon 12a_1b, at 35-37℃ was stabilized by 15-20% sucrose in the medium with 1 M MgCl_2. On the other hand, the virus stability was reduced by 10% polyethylene glycol (PEG, Mol. Wt. 6000) in the medium without MgCl_2, especially when the virus-PEG mixtures were incubated after freeze-drying. Fibrous cellulose CF11 apparently adsorbed majority of the virus and did not change its inactivation rate, when virus-cellulose mixtures were centrifuged and the precipitates were incubated at 37℃. When virus-cellulose mixtures were filtrated through nitrocellulose membranes, however, virus infectivity recovered from the cellulose on the membrane was poor for 0 day's specimen, with apparent stabilization of the adsorbed virus after 1-2 weeks incubation at 37℃. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 30, 号 2, p. 63-71, 発行日 1988-06-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00063-P00071 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 30(2). p63-71, 1988 |