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Production of Slowly Sedimenting and Rapidly Sedimenting Components Associated with Japanese Encephalitis Virus Envelope Glycoprotein E in Infected Cell Culture Fluids
http://hdl.handle.net/10069/4549
http://hdl.handle.net/10069/4549e7df3e5c-e74f-4762-98c0-7d468b6840e4
名前 / ファイル | ライセンス | アクション |
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tm31_03_01_t.pdf (1.8 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | Production of Slowly Sedimenting and Rapidly Sedimenting Components Associated with Japanese Encephalitis Virus Envelope Glycoprotein E in Infected Cell Culture Fluids | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Shameem, Golam Masud Mohammad
× Shameem, Golam Masud Mohammad× Morita, Kouichi× Bundo-Morita, Keiko× Igarashi, Akira |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Production of Japanese encephalitis (JE) virus glycoprotein E into culture fluids of infected mosquito (C6/36) and baby hamster kidney (BHK21) cells at 28 and 37℃ was investigated by the sandwich ELISA using an anti-E monoclonal antibody (MCA). Besides rapidly sedimenting component (RE) at the position of complete virion, slowly sedimenting component (SE) was found near the top of the sucrose gradient sedimentation. Except the first 24 hr of infection when virus production was not so much at 28℃, the peak of SE was more pronounced when the infected cells were incubated at 28 than at 37℃. Production of SE was generally more remarkable in C6/36 than in BHK21 cells at both temperatures. At 28℃, production of both SE and RE continued until 72 hr in C6/36 and 60 hr in BHK21 cells. While, production of both SE and RE declined after 36 hr in both cell lines at 37℃. When SE and RE were recentrifuged, they appeared as independent components. Although both SE and RE were accompanied by the peak of hemagglutination activity (HA), the HA of SE was relatively lower than that of RE by comparing the ELISA color development. Western blotting after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band with apparent Mw of the E protein (54K) for both SE and RE fractions. Electron microscopy (EM) with negative staining revealed spherical particles of 45-55 nm in diameter corresponding to the complete virion together with components of 5-10 nm diameter in RE fractions. While in SE fractions, various components of 5-20 nm in diameter, apparently their aggregates, and membranous structures were observed. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 31, 号 3, p. 111-123, 発行日 1989-09-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00111-P00123 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 31(3). p111-123, 1989 |