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Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System
http://hdl.handle.net/10069/4701
http://hdl.handle.net/10069/47010363a7b6-b092-47db-a5e7-262274949930
名前 / ファイル | ライセンス | アクション |
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tm37_01_04_t.pdf (1.2 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Thayan, Ravindran
× Thayan, Ravindran× Morita, Kouichi× Vijayamalar, Balasubramaniam× Chew, Tong Keong× Sinniah, Mangalam× Igarashi, Akira |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study was carried out to compare the efficiency of two different purification methods used for removing excess primers and short oligonucleotides from PCR products. Our aim was to sequence PCR product directly. The two different purification methods were micro filter spin unit system (Millipore) and Quick Spin Column (Boehringer Mannheim Biochemica). The results indicated that the purification method using microfilter spin unit system was better and yielded better suquencing pattern compared to quick Spin Column. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 37, 号 1, p. 29-33, 発行日 1995-06-23 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00029-P00033 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 37(1). p29-33, 1995 |