WEKO3
アイテム
{"_buckets": {"deposit": "4e832e70-8518-4ec8-b301-72d998277803"}, "_deposit": {"created_by": 2, "id": "2544", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "2544"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00002544", "sets": ["74"]}, "author_link": ["11847", "11845", "11848", "11849", "11846"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-10-25", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "820", "bibliographicPageStart": "814", "bibliographicVolumeNumber": "145", "bibliographic_titles": [{"bibliographic_title": "Journal of Pharmaceutical and Biomedical Analysis"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A fast, accurate, and ultrasensitive high-performance liquid chromatography method with chemiluminescence detection (HPLC-CL) was optimized and validated for the determination of pyrroloquinoline quinone (PQQ) concentration in human plasma following solid-phase extraction (SPE). This method is based on the redox cycle of the reaction between PQQ and dithiothreitol, which generates reactive oxygen species that can be detected using luminol as a CL probe. The isocratic HPLC system comprised an ODS column and 4.0 mM tetra-n-butylammonium bromide in Tris-HNO3 buffer (pH 8.8; 50 mM)-acetonitrile (7:3, v/v) as mobile phase. A novel, rapid, and simple SPE method was also developed providing excellent %recovery (?95.2%) for PQQ from human plasma samples. The proposed method was linear over the range of 4.0?400 nmol/L plasma of PQQ with a lower detection limit (S/N=3) of 1.08 nmol/L plasma (0.27 nM). The method was successfully implemented to determine PQQ concentration in the plasma of healthy individuals after administration of PQQ supplements.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Journal of Pharmaceutical and Biomedical Analysis, 145, pp.814-820; 2017", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier B.V."}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.jpba.2017.08.008", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2017 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "07317085", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fukuda, Mizuho"}], "nameIdentifiers": [{"nameIdentifier": "11845", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "El-Maghrabey, Mahmoud H."}], "nameIdentifiers": [{"nameIdentifier": "11846", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishikawa, Naoya"}], "nameIdentifiers": [{"nameIdentifier": "11847", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikemoto, Kazuto"}], "nameIdentifiers": [{"nameIdentifier": "11848", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroda, Naotaka"}], "nameIdentifiers": [{"nameIdentifier": "11849", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JPBA145_814.pdf", "filesize": [{"value": "590.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 590400.0, "url": {"label": "JPBA145_814.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/2544/files/JPBA145_814.pdf"}, "version_id": "e3df2ab4-9685-4123-9735-7f9259a957b1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Pyrroloquinoline quinone", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HPLC", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chemiluminescence", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Redox cycle", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Human plasma", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone"}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/37761", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-10-25"}, "publish_date": "2019-10-25", "publish_status": "0", "recid": "2544", "relation": {}, "relation_version_is_last": true, "title": ["Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone"], "weko_shared_id": -1}
Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone
http://hdl.handle.net/10069/37761
http://hdl.handle.net/10069/37761cd347c3d-fb81-4db7-9d76-b9b88569077a
名前 / ファイル | ライセンス | アクション |
---|---|---|
JPBA145_814.pdf (590.4 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-10-25 | |||||
タイトル | ||||||
タイトル | Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pyrroloquinoline quinone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPLC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Redox cycle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human plasma | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fukuda, Mizuho
× Fukuda, Mizuho× El-Maghrabey, Mahmoud H.× Kishikawa, Naoya× Ikemoto, Kazuto× Kuroda, Naotaka |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A fast, accurate, and ultrasensitive high-performance liquid chromatography method with chemiluminescence detection (HPLC-CL) was optimized and validated for the determination of pyrroloquinoline quinone (PQQ) concentration in human plasma following solid-phase extraction (SPE). This method is based on the redox cycle of the reaction between PQQ and dithiothreitol, which generates reactive oxygen species that can be detected using luminol as a CL probe. The isocratic HPLC system comprised an ODS column and 4.0 mM tetra-n-butylammonium bromide in Tris-HNO3 buffer (pH 8.8; 50 mM)-acetonitrile (7:3, v/v) as mobile phase. A novel, rapid, and simple SPE method was also developed providing excellent %recovery (?95.2%) for PQQ from human plasma samples. The proposed method was linear over the range of 4.0?400 nmol/L plasma of PQQ with a lower detection limit (S/N=3) of 1.08 nmol/L plasma (0.27 nM). The method was successfully implemented to determine PQQ concentration in the plasma of healthy individuals after administration of PQQ supplements. | |||||
書誌情報 |
Journal of Pharmaceutical and Biomedical Analysis 巻 145, p. 814-820, 発行日 2017-10-25 |
|||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 07317085 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jpba.2017.08.008 | |||||
権利 | ||||||
権利情報 | c 2017 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Pharmaceutical and Biomedical Analysis, 145, pp.814-820; 2017 |