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Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules
http://hdl.handle.net/10069/39423
http://hdl.handle.net/10069/39423a53b7ba7-7200-4cff-8914-f100dc43e59f
名前 / ファイル | ライセンス | アクション |
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CPBul67_772.pdf (419.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-08-29 | |||||
タイトル | ||||||
タイトル | Hydrazide Derivatives of Luminol for Chemiluminescence-Labelling of Macromolecules | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hydrazide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Labelling reagent | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luminol | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shibata, Takayuki
× Shibata, Takayuki× Yoshimura, Hiroki× Yamayoshi, Asako× Tsuda, Nobuaki× Dragusha, Shpend |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A series of chemiluminescent compounds containing a hydrazide group as a nucleophilic functional group has been synthesized. The syntheses were started from chemiluminescent luminol and isoluminol. The linker moiety was easily introduced onto non-nucleophilic exocyclic amino groups of luminol and isoluminol by gentle heating with cyclic acid anhydrides such as glutaric anhydride. The resulting carboxy group was converted to hydrazide by a simple condensation reaction using carbodiimide. Although majority of the synthesized compounds did not emit strong light, a sufficient chemiluminescence intensity was obtained from luminolamido- C2-hydrazide (L2H) comprising of luminol scaffold with a dimethylene linker. The ability of L2H to form a covalent bond with a macromolecule was further investigated by incubation with oxidized horseradish peroxidase. The analysis on matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) MS revealed that the coupling efficiency of L2H was similar to that of commercially available labelling reagent having a hydrazide group. These results suggested that L2H, the luminol hydrazide containing a dimethylene linker, could be useful for the labelling of macromolecules in the sensitive bioassay such as chemiluminescence immunoassay. | |||||
書誌情報 |
Chemical and Pharmaceutical Bulletin 巻 67, 号 8, p. 772-774, 発行日 2019-08-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00092363 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475223 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/cpb.c19-00126 | |||||
権利 | ||||||
権利情報 | c 2019 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Chemical and Pharmaceutical Bulletin, 67(8), pp.772-774; 2019 |