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Reevaluation of the Effect of Iodine on Thyroid Cell Survival and Function Using PCCL3 and Nthy-ori 3-1 Cells
http://hdl.handle.net/10069/40423
http://hdl.handle.net/10069/40423075f55b4-8e47-4b38-ae68-f8b2520301c5
名前 / ファイル | ライセンス | アクション |
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JES4_146.pdf (464.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-04 | |||||
タイトル | ||||||
タイトル | Reevaluation of the Effect of Iodine on Thyroid Cell Survival and Function Using PCCL3 and Nthy-ori 3-1 Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Autophagy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Iodine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NIS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Thyroid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TSHR | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurashige, Tomomi
× Kurashige, Tomomi× Shimamura, Mika× Nagayama, Yuji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The appropriate amount of iodine is critical for normal function of thyroid cells synthesizing thyroid hormones. Although normal thyroid cell lines such as rat PCCL3 and FRTL5 and human Nthy-ori 3-1 have been widely used for in vitro studies on physiological and pathophysiological effects of iodine on thyroid cells, we have recently pointed out the critical differences between FRTL5/PCCL3 cells and Nthy-ori 3-1 cells. Therefore, we here directly compared some of the cellular characteristics?iodine uptake, differentiated status, iodine-induced cytotoxicity, and iodine-regulation of autophagy?between PCCL3 and Nthy-ori 3-1 cells. PCCL3 cells express messenger RNAs for thyrotropin receptor and sodium/iodine symporter and incorporate iodine in a thyrotropin-dependent manner, whereas Nthy-ori 3-1 cells do not either. Nevertheless, both cells were comparably resistant to iodine cytotoxicity: Only far excess iodine (5 × 10?2 M) killed 20% to 40% cells in 24 hours with perchlorate exhibiting no effect, suggesting this cytotoxic effect is due to extracellular iodine. In contrast, a wide range of iodine (5 × 10?9 to 5 × 10?2 M) induced autophagy in PCCL3 cells, which was abolished by perchlorate, indicating intracellular iodine-induction of autophagy, but this effect was not observed in Nthy-ori 3-1 cells. In conclusion,it is critical to discriminate the effect of iodine incorporated into cells from that of extracellular iodine on thyroid cells. Iodine-uptake competent thyroid cells such as PCCL3 and FRTL5 cells, not Nthy-ori 3-1 cells, should be used for studies on iodine effect on thyroid cells. |
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書誌情報 |
Journal of the Endocrine Society 巻 4, 号 11, p. bvaa146, 発行日 2020-10-21 |
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出版者 | ||||||
出版者 | Oxford University Press | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 24721972 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1210/jendso/bvaa146 | |||||
権利 | ||||||
権利情報 | c The Author(s) 2020. Published by Oxford University Press on behalf of the Endocrine Society. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of the Endocrine Society, 4(11), art.no.bvaa146; 2020 |