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Suppression of Peritoneal Fibrosis by Sonoporation of Hepatocyte Growth Factor Gene-Encoding Plasmid DNA in Mice
http://hdl.handle.net/10069/00040519
http://hdl.handle.net/10069/00040519c6815714-1403-429c-a726-1e63cccd20c7
名前 / ファイル | ライセンス | アクション |
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Pharmaceutics13_115.pdf (3.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-02-22 | |||||
タイトル | ||||||
タイトル | Suppression of Peritoneal Fibrosis by Sonoporation of Hepatocyte Growth Factor Gene-Encoding Plasmid DNA in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte growth factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Multicolor deep imaging | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nanobubbles | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Peritoneal fibrosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ultrasound | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nishimura, Koyo
× Nishimura, Koyo× Ogawa, Koki× Kawaguchi, Maho× Fumoto, Shintaro× Mukai, Hidefumi× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Gene therapy is expected to be used for the treatment of peritoneal fibrosis, which is a serious problem associated with long-term peritoneal dialysis. Hepatocyte growth factor (HGF) is a well-known anti-fibrotic gene. We developed an ultrasound and nanobubble-mediated (sonoporation) gene transfection system, which selectively targets peritoneal tissues. Thus, we attempted to treat peritoneal fibrosis by sonoporation-based human HGF (hHGF) gene transfection in mice. To prepare a model of peritoneal fibrosis, mice were intraperitoneally injected with chlorhexidine digluconate. We evaluated the preventive and curative effects of sonoporation-based hHGF transfection by analyzing the following factors: hydroxyproline level, peritoneum thickness, and the peritoneal equilibration test. The transgene expression characteristics of sonoporation were also evaluated using multicolor deep imaging. In early-stage fibrosis in mice, transgene expression by sonoporation was observed in the submesothelial layer. Sonoporation-based hHGF transfection showed not only a preventive effect but also a curative effect for early-stage peritoneal fibrosis. Sonoporation-based hHGF transfection may be suitable for the treatment of peritoneal fibrosis regarding the transfection characteristics of transgene expression in the peritoneum under fibrosis. | |||||
書誌情報 |
Pharmaceutics 巻 13, 号 1, p. 115, 発行日 2021-01-18 |
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出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1999-4923 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/pharmaceutics13010115 | |||||
権利 | ||||||
権利情報 | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pharmaceutics, 13(1), art.no.115, 2021 |