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Attenuated SIRT1 Activity Leads to PER2 Cytoplasmic Localization and Dampens the Amplitude of Bmal1 Promoter-Driven Circadian Oscillation
http://hdl.handle.net/10069/00041007
http://hdl.handle.net/10069/00041007306c932c-bef2-4c10-a473-534c32668f52
名前 / ファイル | ライセンス | アクション |
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FroNeu15-647589.pdf (9.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-11-08 | |||||
タイトル | ||||||
タイトル | Attenuated SIRT1 Activity Leads to PER2 Cytoplasmic Localization and Dampens the Amplitude of Bmal1 Promoter-Driven Circadian Oscillation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | circadian clock | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PER2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NADC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SIRT1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | subcelluar localization | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ashimori, Atsushige
× Ashimori, Atsushige× Nakahata, Yasukazu× Sato, Toshiya× Fukamizu, Yuichiro× Matsui, Takaaki× Yoshitane, Hikari× Fukada, Yoshitaka× Shinohara, Kazuyuki× Bessho, Yasumasa |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The circadian clock possesses robust systems to maintain the rhythm approximately 24 h, from cellular to organismal levels, whereas aging is known to be one of the risk factors linked to the alternation of circadian physiology and behavior. The amount of many metabolites in the cells/body is altered with the aging process, and the most prominent metabolite among them is the oxidized form of nicotinamide adenine dinucleotide (NAD+), which is associated with posttranslational modifications of acetylation and poly-ADP-ribosylation status of circadian clock proteins and decreases with aging. However, how low NAD+ condition in cells, which mimics aged or pathophysiological conditions, affects the circadian clock is largely unknown. Here, we show that low NAD+ in cultured cells promotes PER2 to be retained in the cytoplasm through the NAD+/SIRT1 axis, which leads to the attenuated amplitude of Bmal1 promoter-driven luciferase oscillation. We found that, among the core clock proteins, PER2 is mainly affected in its subcellular localization by NAD+ amount, and a higher cytoplasmic PER2 localization was observed under low NAD+ condition. We further found that NAD+-dependent deacetylase SIRT1 is the regulator of PER2 subcellular localization. Thus, we anticipate that the altered PER2 subcellular localization by low NAD+ is one of the complex changes that occurs in the aged circadian clock. | |||||
書誌情報 |
Frontiers in Neuroscience 巻 15, p. art. no. 647589, 発行日 2021-05-24 |
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出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1662453X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fnins.2021.647589 | |||||
権利 | ||||||
権利情報 | © 2021 Ashimori, Nakahata, Sato, Fukamizu, Matsui, Yoshitane, Fukada, Shinohara and Bessho. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Frontiers in Neuroscience, 15, art. no. 647589; 2021 |