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Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease
http://hdl.handle.net/10069/00041031
http://hdl.handle.net/10069/00041031f6f33cdf-75db-40a6-af5a-cdb129b3b5c3
名前 / ファイル | ライセンス | アクション |
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SCRT12_422.pdf (3.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-11-16 | |||||
タイトル | ||||||
タイトル | Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mesenchymal stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Extracellular vesicles | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ionizing radiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heart disease | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Luo, Lan
× Luo, Lan× Yan, Chen× Fuchi, Naoki× Kodama, Yukinobu× Zhang, Xu× Shinji, Goto× Miura, Kiyonori× Sasaki, Hitoshi× Li, Tao-Sheng |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Radiation-induced heart disease has been reported, but the underlying mechanisms remain unclear. Mesenchymal stem cells (MSCs), also residing in the heart, are highly susceptible to radiation. We examined the hypothesis that the altered secretion of extracellular vesicles (EVs) from MSCs is the trigger of radiation-induced heart disease. Methods: By exposing human placental tissue-derived MSCs to 5 Gy γ-rays, we then isolated EVs from the culture medium 48 h later and evaluated the changes in quantity and quality of EVs from MSCs after radiation exposure. The biological effects of EVs from irradiated MSCs on HUVECs and H9c2 cells were also examined. Results: Although the amount and size distribution of EVs did not differ between the nonirradiated and irradiated MSCs, miRNA sequences indicated many upregulated or downregulated miRNAs in irradiated MSCs EVs. In vitro experiments using HUVEC and H9c2 cells showed that irradiated MSC-EVs decreased cell proliferation (P < 0.01), but increased cell apoptosis and DNA damage. Moreover, irradiated MSC-EVs impaired the HUVEC tube formation and induced calcium overload in H9c2 cells. Conclusions: EVs released from irradiated MSCs show altered miRNA profiles and harmful effects on heart cells, which provides new insight into the mechanism of radiation-related heart disease risks. |
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書誌情報 |
Stem Cell Research & Therapy 巻 12, p. 422, 発行日 2021-07-22 |
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出版者 | ||||||
出版者 | BioMed Central Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1757-6512 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s13287-021-02504-5 | |||||
権利 | ||||||
権利情報 | © The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Stem Cell Research and Therapy, 12, art. no. 422; 2021 |