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A Facile Method for the Quantification of Urinary Uracil Concentration by a Uracil-Specific Fluorescence Derivatization Reaction
http://hdl.handle.net/10069/00041047
http://hdl.handle.net/10069/000410479797dffd-b82e-4f3d-a117-a773bfd393cc
名前 / ファイル | ライセンス | アクション |
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CPBul69_768.pdf (640.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-11-19 | |||||
タイトル | ||||||
タイトル | A Facile Method for the Quantification of Urinary Uracil Concentration by a Uracil-Specific Fluorescence Derivatization Reaction | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ihydropyrimidine dehydrogenase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 5-fluorouracil | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | uracil | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPLC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fluorescence | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shibata, Takayuki
× Shibata, Takayuki× Shimamura, Ryosuke× Yamamoto, Yuji× Sakurai, Hiroki× Fujita, Junya× Yamayoshi, Asako× Nemoto, Toshimitsu× Kabashima, Tsutomu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A facile and reliable fluorescence method for the quantification of urinary uracil concentration is pro-posed herein. The assay utilizes a specific fluorescence (FL) derivatization reaction for uracil using 3-methyl-benzamidoxime as a fluorogenic reagent. Although the presence of urine inhibited the FL reaction, 10 μL of urine was sufficient for the detection of urinary uracil. The uracil derivative was successfully separated from other fluorescent impurities using simple reversed-phase LC with FL detection. Urinary uracil concentra-tions from 16 people were compared with the concentrations obtained by the traditional column-switching liquid chromatographic analysis with UV detection. The FL derivative of uracil appeared as a single peak in the chromatograms of all samples. However, several samples showed an additional peak overlapping the ura-cil peak when using the column-switching method because of UV-active impurities. These results indicated that that the present method is not affected by interfering substances in urine and affords a precise determi-nation of urinary uracil. We expect the proposed method to be applicable for diagnosing dihydropyrimidine dehydrogenase deficiency in 5-fluorouracil chemotherapy. | |||||
書誌情報 |
Chemical and Pharmaceutical Bulletin 巻 69, 号 8, p. 768-772, 発行日 2021-08-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
The Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00092363 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475223 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/cpb.c21-00221 | |||||
権利 | ||||||
権利情報 | © 2021 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Chemical and Pharmaceutical Bulletin, 69(8), pp. 768-772; 2021 |