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Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen
http://hdl.handle.net/10069/00041075
http://hdl.handle.net/10069/000410750b2179fb-deea-40ab-83b2-466c2cf28d6b
名前 / ファイル | ライセンス | アクション |
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IJERPH18_9630.pdf (1.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-12-02 | |||||
タイトル | ||||||
タイトル | Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SARS-CoV-2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nucleocapsid protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IgG indirect ELISA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plaque reduction neutralization test | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mutantu, Pierre Nsele
× Mutantu, Pierre Nsele× Ngwe Tun, Mya Myat× Nabeshima, Takeshi× Yu, Fuxun× Mukadi, Patrick Kakoni× Tanaka, Takeshi× Tashiro, Masato× Fujita, Ayumi× Kanie, Nobuhiro× Oshiro, Ryosaku× Takazono, Takahiro× Imamura, Yoshifumi× Hirayama, Tatsuro× Moi, Meng Ling× Inoue, Shingo× Izumikawa, Koichi× Yasuda, Jiro× Morita, Kouichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). Real-time RT-PCR is the most commonly used method for COVID-19 diagnosis. However, serological assays are urgently needed as complementary tools to RT-PCR. Hachim et al. 2020 and Burbelo et al. 2020 demonstrated that anti-nucleocapsid(N) SARS-CoV-2 antibodies are higher and appear earlier than the spike antibodies. Additionally, cross-reactive antibodies against N protein are more prevalent than those against spike protein. We developed a less cross-reactive immunoglobulin G (IgG) indirect ELISA by using a truncated recombinant SARS-CoV-2 N protein as assay antigen. A highly conserved region of coronaviruses N protein was deleted and the protein was prepared using an E. coli protein expression system. A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay’s performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards. The SARS-CoV-2 N truncated protein-based ELISA showed similar sensitivity (91.1% vs. 91.9%) and specificity (93.8% vs. 93.8%) between the PRNT and spike IgG ELISA, as well as also higher specificity compared to the full-length N protein (93.8% vs. 89.9%). Our ELISA can be used for the diagnosis and surveillance of COVID-19. | |||||
書誌情報 |
International Journal of Environmental Research and Public Health 巻 18, 号 18, p. 9630, 発行日 2021-09-13 |
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出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1660-4601 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/ijerph18189630 | |||||
権利 | ||||||
権利情報 | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Environmental Research and Public Health, 18(18), art. no. 9630; 2021 |