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Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice
http://hdl.handle.net/10069/00041168
http://hdl.handle.net/10069/0004116810ec7bdd-9286-415a-b1e6-575c796b6119
名前 / ファイル | ライセンス | アクション |
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Pharmaceutics13_1810.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-20 | |||||
タイトル | ||||||
タイトル | Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | messenger RNA (mRNA) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mRNA therapeutics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polyplex nanomicelle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | kidney | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | renal pelvis injection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hydrodynamic injection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tubular epithelial cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oyama, Natsuko
× Oyama, Natsuko× Kawaguchi, Maho× Itaka, Keiji× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Renal dysfunction is often associated with the inflammatory cascade, leading to non-reversible nephrofibrosis. Gene therapy has the ability to treat the pathology. However, the difficulty in introducing genes into the kidney, via either viral vectors or plasmid DNA (pDNA), has hampered its extensive clinical use. Messenger RNA (mRNA) therapeutics has recently attracted attention as alternative gene therapies. mRNA allows protein production into post-mitotic cells without the need for transport to the nuclei in the target cells. However, few studies have reported the delivery of mRNA to the kidney. In this study, we attempted to deliver mRNA to the kidney based on the principle of pressure stimulation, by administering mRNA-loaded polyplex nanomicelles via a renal pelvis injection, directly into the kidney. Compared with the administration of naked plasmid DNA (pDNA) and naked mRNA, the mRNA-loaded nanomicelles diffusely induced protein expression in a greater number of cells at the tubular epithelium for some days. The plasma creatinine (Cre) and blood urea nitrogen (BUN) levels after the administration remained similar to those of the sham-operated controls, without marked changes in histological sections. The safety and efficacy of mRNA-loaded nanomicelles would make distinct contributions to the development of mRNA therapeutics for the kidney. | |||||
書誌情報 |
Pharmaceutics 巻 13, 号 11, p. 1810, 発行日 2021-10-29 |
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出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1999-4923 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/pharmaceutics13111810 | |||||
権利 | ||||||
権利情報 | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pharmaceutics, 13(11), art. no. 1810; 2021 |