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Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan
http://hdl.handle.net/10069/00041477
http://hdl.handle.net/10069/000414779b5d2acc-7fc2-4c26-bf23-de96a649f11c
名前 / ファイル | ライセンス | アクション |
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PNTD16_e0010274.pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-04-26 | |||||
タイトル | ||||||
タイトル | Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hashizume, Hiroki
× Hashizume, Hiroki× Taga, Suguru× Sakata, Masayuki K.× Taha, Mahmoud Hussein Mohamed× Siddig, Emmanuel Edwar× Minamoto, Toshifumi× Fahal, Ahmed Hassan× Kaneko, Satoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Mycetoma is a tropical disease caused by several fungi and bacteria present in the soil. Fungal mycetoma and eumycetoma are especially challenging to treat; therefore, prevention, early diagnosis, and early treatment are important, but it is also necessary to understand the geographic distribution of these pathogenic fungi. In this study, we used DNA metabarcoding methodology to identify fungal species from soil samples. Soil sampling was implemented at seven villages in an endemic area of Sennar State in Sudan in 2019, and ten sampling sites were selected in each village according to land-use conditions. In total, 70 soil samples were collected from ground surfaces, and DNA in the soil was extracted with a combined method of alkaline DNA extraction and a commercial soil DNA extraction kit. The region for universal primers was selected to be the ribosomal internal transcribed spacer one region for metabarcoding. After the second PCR for DNA library preparation, the amplicon-based DNA analysis was performed using next-generation sequencing with two sets of universal primers. A total of twelve mycetoma-causative fungal species were identified, including the prime agent, Madurella mycetomatis, and additional pathogens, Falciformispora senegalensis and Falciformispora tompkinsii, in 53 soil samples. This study demonstrated that soil DNA metabarcoding can elucidate the presence of multiple mycetomacausative fungi, which may contribute to accurate diagnosis for patient treatment and geographical mapping. | |||||
書誌情報 |
PLoS Neglected Tropical Diseases 巻 16, 号 3, p. e0010274, 発行日 2022-03-11 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19352727 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pntd.0010274 | |||||
権利 | ||||||
権利情報 | © 2022 Hashizume et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLOS Neglected Tropical Diseases, 16(3), art. no. e0010274; 2022 |