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Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2
http://hdl.handle.net/10069/00041496
http://hdl.handle.net/10069/00041496c904293e-5db0-4b79-8554-a67fd854fa93
名前 / ファイル | ライセンス | アクション |
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viruses14_816.pdf (4.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-05-09 | |||||
タイトル | ||||||
タイトル | Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | marine alkaloid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lamellarin α20-sulfate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | emerging viruses | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antiviral action | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antiviral mechanism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pseudotyped lentiviral vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | molecular dynamics simulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | docking simulation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Izumida, Mai
× Izumida, Mai× Kotani, Osamu× Hayashi, Hideki× Smith, Chris× Fukuda, Tsutomu× Suga, Koushirou× Iwao, Masatomo× Ishibashi, Fumito× Sato, Hironori× Kubo, Yoshinao |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Lamellarin α20-sulfate is a cell-impenetrable marine alkaloid that can suppress infection that is mediated by the envelope glycoprotein of human immunodeficiency virus type 1. We explored the antiviral action and mechanisms of this alkaloid against emerging enveloped RNA viruses that use endocytosis for infection. The alkaloid inhibited the infection of retroviral vectors that had been pseudotyped with the envelope glycoprotein of Ebola virus and SARS-CoV-2. The antiviral effects of lamellarin were independent of the retrovirus Gag-Pol proteins. Interestingly, although heparin and dextran sulfate suppressed the cell attachment of vector particles, lamellarin did not. In silico structural analyses of the trimeric glycoprotein of the Ebola virus disclosed that the principal lamellarin-binding site is confined to a previously unappreciated cavity near the NPC1-binding site and fusion loop, whereas those for heparin and dextran sulfate were dispersed across the attachment and fusion subunits of the glycoproteins. Notably, lamellarin binding to this cavity was augmented under conditions where the pH was 5.0. These results suggest that the final action of the alkaloid against Ebola virus is specific to events following endocytosis, possibly during conformational glycoprotein changes in the acidic environment of endosomes. Our findings highlight the unique biological and physicochemical features of lamellarin α20-sulfate and should lead to the further use of broadly reactive antivirals to explore the structural mechanisms of virus replication. | |||||
書誌情報 |
Viruses 巻 14, 号 4, p. 816, 発行日 2022-04-15 |
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出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19994915 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/v14040816 | |||||
権利 | ||||||
権利情報 | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Viruses, 14(4), art. no. 816; 2022 |