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A screen of FDA-approved drugs with minigenome identified tigecycline as an antiviral targeting nucleoprotein of CrimeanCongo hemorrhagic fever virus
http://hdl.handle.net/10069/00041511
http://hdl.handle.net/10069/000415113814083e-f859-436e-ae2b-ca4854488c38
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AR200_105276.pdf (1.2 MB)
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220205_Supplemental data1_Result of Drug screening.xlsx (129.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-05-12 | |||||
タイトル | ||||||
タイトル | A screen of FDA-approved drugs with minigenome identified tigecycline as an antiviral targeting nucleoprotein of CrimeanCongo hemorrhagic fever virus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Crimean-Congo hemorrhagic fever virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Minigenome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Drug screening | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tigecycline | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nucleoprotein | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hirano, Minato
× Hirano, Minato× Sakurai, Yasuteru× Urata, Shuzo× Kurosaki, Yohei× Yasuda, Jiro× Yoshii, Kentaro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Crimean-Congo hemorrhagic fever virus (CCHFV) belongs to the genus Orthonairovirus and is the causative agent of a viral hemorrhagic disease with a case fatality rate of 30%. However, limited studies have been conducted to explore antiviral compounds specific to CCHFV. In this study, we developed a minigenome system of orthonairoviruses, CCHFV and Hazara virus to analyze viral replication and screened an FDA-approved compound library. The transfection of the minigenome components induced marked increase in luciferase expression, indicating the sufficient replication and translation of reporter RNA. Compound library screening identified 14 candidate compounds that significantly decreased luciferase activity. Some of the compounds also inhibited the replication of the infectious Hazara virus. The mechanism of inhibition by tigecycline was further analyzed, and a decrease in the interaction between the viral N protein and RNA by tigecycline was observed. This work provides a basis for validation using animal models and the design of chemical derivatives with stronger activity in future studies on the development of an antiviral against CCHFV. | |||||
書誌情報 |
Antiviral Research 巻 200, p. 105276, 発行日 2022-03-10 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01663542 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.antiviral.2022.105276 | |||||
権利 | ||||||
権利情報 | © 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Antiviral Research, 200, art. no. 105276; 2022 |